Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/76365
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Type: Journal article
Title: Individual and population quantitative analyses of calcium flux in T-cells activated on functionalized material surfaces
Author: Christo, S.
Sarvestani, G.
Griesser, S.
Coad, B.
Griesser, H.
Vasilev, K.
Brown, M.
Diener, K.
Hayball, J.
Citation: Australian Journal of Chemistry, 2012; 65(1):45-49
Publisher: C S I R O Publishing
Issue Date: 2012
ISSN: 0004-9425
Statement of
Responsibility: 
Susan N. Christo, Ghafar. T. Sarvestani, Stefani S. Griesser, Bryan R. Coad, Hans J. Griesser, Krasimir Vasilev, Michael P. Brown, Kerrilyn R. Diener and John D. Hayball
Abstract: We have developed a novel method for activating T-cells on material surfaces that enable individual and population-based analyses of intracellular calcium flux, as a quantitative measure of T-cell receptor engagement. Functionalized material surfaces were created using a plasma-polymerized foundation layer to immobilize stimulatory T-cell ligands, which could induce T-cell receptor-dependent calcium flux in naive T-cells. Real-time confocal microscopic detection and quantification of calcium flux using paired fluorescent ratiometric probes facilitated the tracking and analysis of response profiles of individual T-cells, as well as population analyses using a combination of individual T-cell events. This type of combined analysis cannot be achieved using traditional population-based flow cytometric approaches, and thus provides a logical step towards developing the capacity to assess the magnitude and quality of inherently heterogeneous effector T-cell responses to antigenic challenge.
Rights: Copyright status unknown
RMID: 0020116242
DOI: 10.1071/CH11311
Appears in Collections:Medicine publications

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