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https://hdl.handle.net/2440/76365
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Type: | Journal article |
Title: | Individual and population quantitative analyses of calcium flux in T-cells activated on functionalized material surfaces |
Author: | Christo, S. Sarvestani, G. Griesser, S. Coad, B. Griesser, H. Vasilev, K. Brown, M. Diener, K. Hayball, J. |
Citation: | Australian Journal of Chemistry: an international journal for chemical science, 2012; 65(1):45-49 |
Publisher: | C S I R O Publishing |
Issue Date: | 2012 |
ISSN: | 0004-9425 |
Statement of Responsibility: | Susan N. Christo, Ghafar. T. Sarvestani, Stefani S. Griesser, Bryan R. Coad, Hans J. Griesser, Krasimir Vasilev, Michael P. Brown, Kerrilyn R. Diener and John D. Hayball |
Abstract: | We have developed a novel method for activating T-cells on material surfaces that enable individual and population-based analyses of intracellular calcium flux, as a quantitative measure of T-cell receptor engagement. Functionalized material surfaces were created using a plasma-polymerized foundation layer to immobilize stimulatory T-cell ligands, which could induce T-cell receptor-dependent calcium flux in naive T-cells. Real-time confocal microscopic detection and quantification of calcium flux using paired fluorescent ratiometric probes facilitated the tracking and analysis of response profiles of individual T-cells, as well as population analyses using a combination of individual T-cell events. This type of combined analysis cannot be achieved using traditional population-based flow cytometric approaches, and thus provides a logical step towards developing the capacity to assess the magnitude and quality of inherently heterogeneous effector T-cell responses to antigenic challenge. |
Rights: | Copyright status unknown |
DOI: | 10.1071/CH11311 |
Appears in Collections: | Aurora harvest 4 Medicine publications |
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