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Type: Journal article
Title: Barramundi (Lates calcarifer) desaturase with Δ6/Δ8 dual activities
Other Titles: Barramundi (Lates calcarifer) desaturase with Delta 6/Delta 8 dual activities
Author: Tu, W.
Cook-Johnson, R.
James, M.
Muhlhausler, B.
Stone, D.
Gibson, R.
Citation: Biotechnology Letters, 2012; 34(7):1283-1296
Publisher: Kluwer Academic Publ
Issue Date: 2012
ISSN: 0141-5492
Statement of
Wei-Chun Tu, Rebecca J. Cook-Johnson, Michael J. James, Beverly S. Mühlhäusler, David A. J. Stone, Robert A. Gibson
Abstract: Barramundi is a commercially farmed fish in Australia. To examine the potential for barramundi to metabolise dietary α-linolenic acid (ALA, 18:3 n-3), the existence of barramundi desaturase enzymes was examined. A putative fatty acid Δ6 desaturase was cloned from barramundi liver and expressed in yeast. Functional expression revealed Δ6 desaturase activity with both the 18 carbon (C18) and C24 n-3 fatty acids, ALA and 24:5 n-3 as well as the C18 n-6 fatty, linoleic acid (LA, 18:2 n-6). Metabolism of ALA was favoured over LA. The enzyme also had Δ8 desaturase activity which raises the potential for synthesis in barramundi of omega-3 (n-3) long chain polyunsaturated fatty acids from ALA via a pathway that bypasses the initial Δ6 desaturase step. Our findings not only provide molecular evidence for the fatty acid desaturation pathway in the barramundi but also highlight the importance of taking extracellular fatty acid levels into account when assessing enzyme activity expressed in Saccharomyces cerevisiae.
Keywords: Barramundi; Δ6 desaturase; Extracellular fatty acids; α-Linolenic acid; Lipid metabolism; n-3 LCPUFA
Rights: © Springer Science+Business Media B.V. 2012
RMID: 0020120035
DOI: 10.1007/s10529-012-0891-x
Appears in Collections:Agriculture, Food and Wine publications

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