Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/76951
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Type: Journal article
Title: A physical, genetic and functional sequence assembly of the barley genome
Author: Langridge, P.
Shi, B.
Fincher, G.
Citation: Nature, 2012; 491(7426):711-717
Publisher: Nature Publishing Group
Issue Date: 2012
ISSN: 0028-0836
1476-4687
Contributor: Langridge, Peter
Shi, Bu-jun
Fincher, Geoffrey
Statement of
Responsibility: 
The International Barley Genome Sequencing Consortium
Abstract: Barley (Hordeum vulgare L.) is among the world’s earliest domesticated and most important crop plants. It is diploid with a large haploid genome of 5.1 gigabases (Gb). Here we present an integrated and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a structured whole-genome context. We developed a physical map of 4.98 Gb, with more than 3.90 Gb anchored to a high-resolution genetic map. Projecting a deep whole-genome shotgun assembly, complementary DNA and deep RNA sequence data onto this framework supports 79,379 transcript clusters, including 26,159 ‘high-confidence’ genes with homology support from other plant genomes. Abundant alternative splicing, premature termination codons and novel transcriptionally active regions suggest that post-transcriptional processing forms an important regulatory layer. Survey sequences from diverse accessions reveal a landscape of extensive single-nucleotide variation. Our data provide a platform for both genome-assisted research and enabling contemporary crop improvement.
Keywords: International Barley Genome Sequencing Consortium
Hordeum
Crops, Agricultural
Codon, Nonsense
Physical Chromosome Mapping
Sequence Analysis, DNA
Genomics
Evolution, Molecular
Gene Expression Regulation, Plant
Alternative Splicing
Repetitive Sequences, Nucleic Acid
Polymorphism, Single Nucleotide
Genes, Plant
Genome, Plant
Molecular Sequence Annotation
Transcriptome
Description: University of Adelaide contributors: Peter Langridge & Bu-jun Shi for Australian Centre for Plant Functional Genomics, Geoffrey Fincher for ARC Centre of Excellence in Plant Cell Walls
Rights: This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
DOI: 10.1038/nature11543
Appears in Collections:Agriculture, Food and Wine publications
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