Please use this identifier to cite or link to this item:
http://hdl.handle.net/2440/76951
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Type: | Journal article |
Title: | A physical, genetic and functional sequence assembly of the barley genome |
Author: | Langridge, P. Shi, B. Fincher, G. |
Citation: | Nature, 2012; 491(7426):711-717 |
Publisher: | Nature Publishing Group |
Issue Date: | 2012 |
ISSN: | 0028-0836 1476-4687 |
Contributor: | Langridge, Peter Shi, Bu-jun Fincher, Geoffrey |
Statement of Responsibility: | The International Barley Genome Sequencing Consortium |
Abstract: | Barley (Hordeum vulgare L.) is among the world’s earliest domesticated and most important crop plants. It is diploid with a large haploid genome of 5.1 gigabases (Gb). Here we present an integrated and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a structured whole-genome context. We developed a physical map of 4.98 Gb, with more than 3.90 Gb anchored to a high-resolution genetic map. Projecting a deep whole-genome shotgun assembly, complementary DNA and deep RNA sequence data onto this framework supports 79,379 transcript clusters, including 26,159 ‘high-confidence’ genes with homology support from other plant genomes. Abundant alternative splicing, premature termination codons and novel transcriptionally active regions suggest that post-transcriptional processing forms an important regulatory layer. Survey sequences from diverse accessions reveal a landscape of extensive single-nucleotide variation. Our data provide a platform for both genome-assisted research and enabling contemporary crop improvement. |
Keywords: | International Barley Genome Sequencing Consortium; Hordeum; Crops, Agricultural; Codon, Nonsense; Physical Chromosome Mapping; Sequence Analysis, DNA; Genomics; Evolution, Molecular; Gene Expression Regulation, Plant; Alternative Splicing; Repetitive Sequences, Nucleic Acid; Polymorphism, Single Nucleotide; Genes, Plant; Genome, Plant; Molecular Sequence Annotation; Transcriptome |
Description: | University of Adelaide contributors: Peter Langridge & Bu-jun Shi for Australian Centre for Plant Functional Genomics, Geoffrey Fincher for ARC Centre of Excellence in Plant Cell Walls |
Rights: | This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ |
RMID: | 0020123304 |
DOI: | 10.1038/nature11543 |
Appears in Collections: | Agriculture, Food and Wine publications |
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hdl_76951.pdf | Published version | 1.16 MB | Adobe PDF | View/Open |
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