Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/7740
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Type: Journal article
Title: Multiple neurotrophic factors including NGF-like activity in nerve regeneration chamber fluids
Author: Bates, D.
Mangelsdorf, D.
Ridings, J.
Citation: Neurochemistry International: the journal for the publication of cellular and molecular aspects of neurochemistry, 1995; 26(3):281-293
Publisher: Pergamon Press
Issue Date: 1995
ISSN: 0197-0186
1872-9754
Abstract: Silicone nerve regeneration chambers were implanted between the cut ends of the sciatic nerve of adult rats. Neurotrophic activities in cell-free fluids collected from the chambers were determined using bioassays for survival of embryonic chick ciliary and sympathetic neurons in culture. Separation by molecular exclusion HPLC of the components of fluids collected 1, 2 or 3 days after implantation revealed the presence of a multitude of neurotrophic factors differing in their molecular weights, specificity towards the two types of neurons, and time course. Antiserum to nerve growth factor partially blocked sympathetic activity of fluids collected at 1 day. Affinity purified antibody was also effective and completely eliminated bioactivity of HPLC fractions corresponding to the molecular weight of nerve growth factor. The presence in the fluids of 13-18 and 20-32 kD components active towards ciliary neurons is consistent with the release of fibroblast growth factor and ciliary neurotrophic factor respectively. The stimulation of sympathetic neurons by the 13-18 kD material, and also by 4-6 and 7-11 kD components cannot be entirely accounted for by known factors. This study demonstrates that a number of neurotrophic factors, which differ in their specificity towards sympathetic and parasympathetic neurons, are made available to the region of axonal regrowth over the first few days of regeneration. Contrary to earlier reports, nerve growth factor-like activity was shown to be present in nerve regeneration chambers.
Keywords: Sciatic Nerve
Body Fluids
Chick Embryo
Animals
Rats
Rats, Wistar
Nerve Growth Factors
Biological Assay
Chromatography, High Pressure Liquid
Prostheses and Implants
Nerve Regeneration
Molecular Weight
Female
DOI: 10.1016/0197-0186(94)00126-F
Appears in Collections:Aurora harvest 4
Paediatrics publications

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