Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/77466
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Type: Journal article
Title: Endo-(1,4)-β-Glucanase gene families in the grasses: temporal and spatial Co-transcription of orthologous genes
Other Titles: Endo-(1,4)-beta-Glucanase gene families in the grasses: temporal and spatial Co-transcription of orthologous genes
Author: Buchanan, M.
Burton, R.
Dhugga, K.
Rafalski, A.
Tingey, S.
Shirley, N.
Fincher, G.
Citation: BMC Plant Biology, 2012; 12(1):1-19
Publisher: BioMed Central Ltd.
Issue Date: 2012
ISSN: 1471-2229
1471-2229
Organisation: Australian Centre for Plant Functional Genomics (ACPFG)
Statement of
Responsibility: 
Margaret Buchanan, Rachel A Burton, Kanwarpal S Dhugga, Antoni J Rafalski, Scott V Tingey, Neil J Shirley and Geoffrey B Fincher
Abstract: BACKGROUND Endo-(1,4)-β-glucanase (cellulase) glycosyl hydrolase GH9 enzymes have been implicated in several aspects of cell wall metabolism in higher plants, including cellulose biosynthesis and degradation, modification of other wall polysaccharides that contain contiguous (1,4)-β-glucosyl residues, and wall loosening during cell elongation. RESULTS The endo-(1,4)-β-glucanase gene families from barley (Hordeum vulgare), maize (Zea mays), sorghum (Sorghum bicolor), rice (Oryza sativa) and Brachypodium (Brachypodium distachyon) range in size from 23 to 29 members. Phylogenetic analyses show variations in clade structure between the grasses and Arabidopsis, and indicate differential gene loss and gain during evolution. Map positions and comparative studies of gene structures allow orthologous genes in the five species to be identified and synteny between the grasses is found to be high. It is also possible to differentiate between homoeologues resulting from ancient polyploidizations of the maize genome. Transcript analyses using microarray, massively parallel signature sequencing and quantitative PCR data for barley, rice and maize indicate that certain members of the endo-(1,4)-β-glucanase gene family are transcribed across a wide range of tissues, while others are specifically transcribed in particular tissues. There are strong correlations between transcript levels of several members of the endo-(1,4)-β-glucanase family and the data suggest that evolutionary conservation of transcription exists between orthologues across the grass family. There are also strong correlations between certain members of the endo-(1,4)-β-glucanase family and other genes known to be involved in cell wall loosening and cell expansion, such as expansins and xyloglucan endotransglycosylases. CONCLUSIONS The identification of these groups of genes will now allow us to test hypotheses regarding their functions and joint participation in wall synthesis, re-modelling and degradation, together with their potential role in lignocellulose conversion during biofuel production from grasses and cereal crop residues.
Keywords: Biofuels; Cell walls; Cellulases; Cellulose synthesis; Co-expression; Grasses; Stem strength
Description: Extent: 19p.
Rights: © 2012 Buchanan et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
RMID: 0020125632
DOI: 10.1186/1471-2229-12-235
Appears in Collections:Australian Centre for Plant Functional Genomics publications

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