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Type: Journal article
Title: Plant carbohydrate binding module enhances activity of hybrid microbial cellulase enzyme
Author: Byrt, C.
Cahyanegara, R.
Grof, C.
Citation: Frontiers in Plant Science, 2012; 3(254):1-8
Publisher: Frontiers Research Foundation
Issue Date: 2012
ISSN: 1664-462X
Statement of
Caitlin S. Byrt, Ricky Cahyanegara and Christopher P.L. Grof
Abstract: A synthetic, highly active cellulase enzyme suitable for in planta production may be a valuable tool for biotechnological approaches to develop transgenic biofuel crops with improved digestibility. Here, we demonstrate that the addition of a plant derived carbo-hydrate binding module (CBM) to a synthetic glycosyl hydrolase improved the activity of the hydrolase in releasing sugar from plant biomass. A CEL-HYB1-CBM enzyme was generated by fusing a hybrid microbial cellulase, CEL-HYB1, with the CBM of the tomato (Solanum lycopersicum) SlCel9C1 cellulase. CEL-HYB1 and CEL-HYB1-CBM enzymes were produced in vitro using Pichia pastoris and the activity of these enzymes was tested using carboxymethylcellulose, MUC, and native crystalline cellulose assays. The presence of the CBM substantially improved the endoglucanase activity of CEL-HYB1, especially against the native crystalline cellulose encountered in Sorghum bicolor plant cell walls. These results indicate that addition of an endogenous plant derived CBM to cellulase enzymes may enhance hydrolytic activity.
Keywords: Cellulase binding module
glycosyl hydrolase
Description: Extent: 8 p.
Rights: Copyright: © 2012 Byrt, Cahyanegara and Grof. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.
DOI: 10.3389/fpls.2012.00254
Appears in Collections:Agriculture, Food and Wine publications
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