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Type: Journal article
Title: The brn-2 gene regulates the melanocytic phenotype and tumorigenic potential of human melanoma cells
Author: Angus, J.
Thompson, F.
Murphy, K.
Baker, E.
Sutherland, G.
Parsons, P.
Sturm, R.
Citation: Oncogene, 1995; 11(4):691-700
Publisher: Macmillan Press
Issue Date: 1995
ISSN: 0950-9232
Abstract: The Oct transcription factors N-Oct-3 and N-Oct-5 are differentially expressed in normal melanocytes, melanoma tumors and cell lines. We have cloned the human brn-2 gene and have shown that it encodes both the N-Oct-3 and N-Oct-5 octamer binding activities detected in melanoma cells. The brn-2 genomic locus has been mapped to chromosome 6q16 and although chromosomal aberrations are common in this region in melanoma, no deletion or rearrangement of the brn-2 gene in melanoma cell lines was observed. Sequencing of the entire gene showed that there are no intervening sequences within the open reading frame. Antisense RNA-mediated inhibition of brn-2 gene expression in melanoma cells was associated with a change in morphology and loss of melanocytic and neural crest markers, including the melanocyte transcription factor microphthalmia and the TYRP pigmentation genes. In addition, loss of brn-2 in these cells resulted in the complete loss of ability to form tumors in SCID and nu/nu mice. These results suggest roles for brn-2 in the determination of the melanocytic lineage and in the tumorigenic phenotype of melanoma.
Keywords: Cell Line
Tumor Cells, Cultured
Chromosomes, Human, Pair 6
Mice, SCID
Chromosome Deletion
Homeodomain Proteins
Intercellular Adhesion Molecule-1
Transcription Factors
RNA, Antisense
DNA, Neoplasm
Blotting, Southern
In Situ Hybridization, Fluorescence
Restriction Mapping
Cell Differentiation
Gene Expression
Gene Expression Regulation, Neoplastic
Base Sequence
Molecular Sequence Data
POU Domain Factors
Appears in Collections:Aurora harvest 4
Paediatrics publications

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