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dc.contributor.authorAngus, J.-
dc.contributor.authorThompson, F.-
dc.contributor.authorMurphy, K.-
dc.contributor.authorBaker, E.-
dc.contributor.authorSutherland, G.-
dc.contributor.authorParsons, P.-
dc.contributor.authorSturm, R.-
dc.identifier.citationOncogene, 1995; 11(4):691-700-
dc.description.abstractThe Oct transcription factors N-Oct-3 and N-Oct-5 are differentially expressed in normal melanocytes, melanoma tumors and cell lines. We have cloned the human brn-2 gene and have shown that it encodes both the N-Oct-3 and N-Oct-5 octamer binding activities detected in melanoma cells. The brn-2 genomic locus has been mapped to chromosome 6q16 and although chromosomal aberrations are common in this region in melanoma, no deletion or rearrangement of the brn-2 gene in melanoma cell lines was observed. Sequencing of the entire gene showed that there are no intervening sequences within the open reading frame. Antisense RNA-mediated inhibition of brn-2 gene expression in melanoma cells was associated with a change in morphology and loss of melanocytic and neural crest markers, including the melanocyte transcription factor microphthalmia and the TYRP pigmentation genes. In addition, loss of brn-2 in these cells resulted in the complete loss of ability to form tumors in SCID and nu/nu mice. These results suggest roles for brn-2 in the determination of the melanocytic lineage and in the tumorigenic phenotype of melanoma.-
dc.publisherMacmillan Press-
dc.subjectCell Line-
dc.subjectTumor Cells, Cultured-
dc.subjectChromosomes, Human, Pair 6-
dc.subjectMice, SCID-
dc.subjectChromosome Deletion-
dc.subjectHomeodomain Proteins-
dc.subjectIntercellular Adhesion Molecule-1-
dc.subjectTranscription Factors-
dc.subjectRNA, Antisense-
dc.subjectDNA, Neoplasm-
dc.subjectBlotting, Southern-
dc.subjectIn Situ Hybridization, Fluorescence-
dc.subjectRestriction Mapping-
dc.subjectCell Differentiation-
dc.subjectGene Expression-
dc.subjectGene Expression Regulation, Neoplastic-
dc.subjectBase Sequence-
dc.subjectMolecular Sequence Data-
dc.subjectPOU Domain Factors-
dc.titleThe brn-2 gene regulates the melanocytic phenotype and tumorigenic potential of human melanoma cells-
dc.typeJournal article-
Appears in Collections:Aurora harvest 4
Paediatrics publications

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