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dc.contributor.authorTran, H.en
dc.contributor.authorLewis, M.en
dc.contributor.authorTan, L.W.en
dc.contributor.authorLester, S.en
dc.contributor.authorBaker, L.en
dc.contributor.authorNg, J.en
dc.contributor.authorHamilton-Bruce, M.en
dc.contributor.authorHill, C.en
dc.contributor.authorKoblar, S.en
dc.contributor.authorRischmueller, M.en
dc.contributor.authorRuffin, R.en
dc.contributor.authorWormald, P.J.en
dc.contributor.authorZalewski, P.en
dc.contributor.authorLang, C.en
dc.identifier.citationJournal of Allergy, 2012; Online(819176):1-13en
dc.descriptionExtent: 18p.en
dc.description.abstractLittle is known about innate immunity and components of inflammasomes in airway epithelium. This study evaluated immunohistological evidence for NLRP3 inflammasomes in normal and inflamed murine (Balb/c) airway epithelium in a model of ovalbumin (OVA) induced allergic airway inflammation. The airway epithelium of control mice exhibited strong cytoplasmic staining for total caspase-1, ASC, and NLRP3, whereas the OVA mice exhibited strong staining for active caspase-1, with redistribution of caspase-1, IL-1β and IL-18, indicating possible activation of the NLRP3 inflammasome. Active caspase-1, NLRP3, and other inflammasome components were also detected in tissue eosinophils from OVA mice, and may potentially contribute to IL-1β and IL-18 production. In whole lung, inRNA expression of NAIP and procaspase-1 was increased in OVA mice, whereas NLRP3, IL-1β and IL-18 decreased. Some OVA-treated mice also had significantly elevated and tightly correlated serum levels of IL-1β and TNFα. In cultured normal human bronchial epithelial cells, LPS priming resulted in a significant increase in NLRP3 and II-lp protein expression. This study is the first to demonstrate NLRP3 inflammasome components in normal airway epithelium and changes with inflammation. We propose activation and/or luminal release of the inflammasome is a feature of allergic airway inflammation which may contribute to disease pathogenesis.en
dc.description.statementofresponsibilityHai B. Tran, Martin D. Lewis, LorWai Tan, Susan E. Lester, Leonie M. Baker, Jia Ng, Monica A. Hamilton-Bruce, Catherine L. Hill, Simon A. Koblar, Maureen Rischmueller, Richard E. Ruffin, Peter J. Wormald, Peter D. Zalewski, and Carol J. Langen
dc.publisherHindawi Publishing Corporationen
dc.rightsCopyright © 2012 Hai B. Tran et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.titleImmunolocalization of NLRP3 inflammasome in normal murine airway epithelium and changes following induction of ovalbumin-induced airway inflammationen
dc.typeJournal articleen
pubs.library.collectionSurgery publicationsen
dc.identifier.orcidTran, H. [0000-0002-9463-4033]en
dc.identifier.orcidLewis, M. [0000-0002-3332-3776]en
dc.identifier.orcidLester, S. [0000-0003-3013-2701]en
dc.identifier.orcidBaker, L. [0000-0003-2498-1133]en
dc.identifier.orcidNg, J. [0000-0003-2302-2643]en
dc.identifier.orcidHamilton-Bruce, M. [0000-0002-5222-620X]en
dc.identifier.orcidHill, C. [0000-0001-8289-4922]en
dc.identifier.orcidKoblar, S. [0000-0002-8667-203X]en
dc.identifier.orcidRischmueller, M. [0000-0001-5057-3286]en
dc.identifier.orcidWormald, P.J. [0000-0001-7753-7277]en
dc.identifier.orcidZalewski, P. [0000-0001-5196-2611]en
dc.identifier.orcidLang, C. [0000-0002-3832-6984]en
Appears in Collections:Surgery publications

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