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Type: Book chapter
Title: Transcriptomics on small samples
Author: Roy, S.
Conn, S.
Mayo, G.
Athman, A.
Gilliham, M.
Citation: Plant Salt Tolerance: Methods and Protocols, 2012 / Shabala, S., Cuin, T. (ed./s), vol.913, pp.335-350
Publisher: Springer
Publisher Place: New York
Issue Date: 2012
Series/Report no.: Methods in Molecular Biology; 913
ISBN: 9781617799853
Editor: Shabala, S.
Cuin, T.
Statement of
Stuart J. Roy, Simon J. Conn, Gwenda M. Mayo, Asmini Athman, and Matthew Gilliham
Abstract: Interrogating the cell-specific transcriptome forms an important component of understanding the role that specific cells play in assisting a plant to overcome abiotic stress. Among the challenges arising when extracting RNA from individual plant cells are: the isolation of pure cell populations; the small yield of material when isolating specific cell types, and ensuring an accurate representation of the transcriptome from each cell type after amplification of RNA. Here we describe two approaches for isolating RNA from specific cell types—single cell sampling and analysis (SiCSA) and laser capture microdissection. Isolated RNA can then be directly sampled qualitatively using reverse transcription PCR (RT-PCR) or amplified for profiling ¬multiple specific genes using quantitative RT-PCR and genome-wide transcript analyses.
Keywords: Plants
Plant Roots
RNA, Plant
Gene Expression Profiling
Single-Cell Analysis
Laser Capture Microdissection
Rights: © Springer Science+Business Media, LLC 2012
DOI: 10.1007/978-1-61779-986-0_22
Published version:
Appears in Collections:Agriculture, Food and Wine publications
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