Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/78053
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Type: Journal article
Title: In vitro propagation of Phebalium equestre and P. hillebrandii (Rutaceae)
Author: Jusaitis, M.
Citation: In Vitro Cellular and Developmental Biology. Plant, 1995; 31(3):140-143
Publisher: Society for In Vitro Biology
Issue Date: 1995
ISSN: 1054-5476
1475-2689
Statement of
Responsibility: 
Manfred Jusaitis
Abstract: The endangered Phebalium equestre D. A. Cooke and the rare Phebalium hillebrandii J. H. Willis were propagated in vitro using shoot tips and nodal segments as explants. For each species, shoot proliferation was initiated on de Fossard MZZM (Medium levels of minerals, Zero auxins, Zero cytokinins and Medium levels of sucrose, growth factors, and amino acids) medium supplemented with 1 µM benzyladenine. Excised P. equestre shoots initiated roots when cultured on MZZM medium containing 60 µM 2,4-dichlorophenoxyacetic acid, while P. hillebrandii shoots required LZZL (Low levels of minerals, Zero auxins, Zero cytokinins and Low levels of sucrose, growth factors, and amino acids) medium containing 10 µM 2,4-D for maximal root initiation. Both species required transfer to MZZM medium without growth regulators after 2 wk to allow root initials to develop and grow. Plantlets were successfully transferred to soil with 80% survival after 2 mo.
Keywords: Phebalium; endangered plant; ex situ conservation; micropropagation; tissue culture.
Rights: © 1995 Society for In Vitro Biology
RMID: 0030000528
DOI: 10.1007/BF02632009
Published version: http://www.jstor.org/stable/4293077
Appears in Collections:Earth and Environmental Sciences publications

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