Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/78053
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dc.contributor.authorJusaitis, M.en
dc.date.issued1995en
dc.identifier.citationIn Vitro Cellular and Developmental Biology. Plant, 1995; 31(3):140-143en
dc.identifier.issn1054-5476en
dc.identifier.issn1475-2689en
dc.identifier.urihttp://hdl.handle.net/2440/78053-
dc.description.abstractThe endangered Phebalium equestre D. A. Cooke and the rare Phebalium hillebrandii J. H. Willis were propagated in vitro using shoot tips and nodal segments as explants. For each species, shoot proliferation was initiated on de Fossard MZZM (Medium levels of minerals, Zero auxins, Zero cytokinins and Medium levels of sucrose, growth factors, and amino acids) medium supplemented with 1 µM benzyladenine. Excised P. equestre shoots initiated roots when cultured on MZZM medium containing 60 µM 2,4-dichlorophenoxyacetic acid, while P. hillebrandii shoots required LZZL (Low levels of minerals, Zero auxins, Zero cytokinins and Low levels of sucrose, growth factors, and amino acids) medium containing 10 µM 2,4-D for maximal root initiation. Both species required transfer to MZZM medium without growth regulators after 2 wk to allow root initials to develop and grow. Plantlets were successfully transferred to soil with 80% survival after 2 mo.en
dc.description.statementofresponsibilityManfred Jusaitisen
dc.language.isoenen
dc.publisherSociety for In Vitro Biologyen
dc.rights© 1995 Society for In Vitro Biologyen
dc.source.urihttp://www.jstor.org/stable/4293077en
dc.subjectPhebalium; endangered plant; ex situ conservation; micropropagation; tissue culture.en
dc.titleIn vitro propagation of Phebalium equestre and P. hillebrandii (Rutaceae)en
dc.typeJournal articleen
dc.identifier.rmid0030000528en
dc.identifier.doi10.1007/BF02632009en
dc.identifier.pubid64534-
pubs.library.collectionEarth and Environmental Sciences publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidJusaitis, M. [0000-0002-0313-9636]en
Appears in Collections:Earth and Environmental Sciences publications

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