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Type: Conference paper
Title: Reflectometric interference microchip based on nanoporous alumina for label-free detection of circulating tumour cells
Author: Kumeria, T.
Kurkuri, M.
Diener, K.
Parkinson, L.
Losic, D.
Citation: APMC10 10th Asia-Pacific Microscopy Conference, ICONN2012 2012 International Conference on Nanoscience and Nanotechnology, ACMM22 2012 22nd Australian Conference on Microscopy and Microanalysis, Conference Proceedings / B. Griffin, L. Faraone, M. Martyniuk (eds.): pp.746-1-746-3
Issue Date: 2012
ISBN: 9781740522458
Conference Name: International Conference on Nanoscience and Nanotechnology (2012 : Perth, Western Australia)
Statement of
T. Kumeria, M. D. Kurkuri, K. Diener, L. Parkinson and D. Losic
Abstract: Circulating tumor cells (CTCs) are defined as circulating tumor cells in the peripheral blood that shed from either the primary tumors or its metastases. In this study, we aim to materialize a new real-time, label-free microchip biosensor for detection of circulating tumour cells (CTC) based on reflectometric interference spectroscopy (RIfS) on nanoporous anodic aluminium oxide (AAO). Biotinylated human anti-EpCAM antibody that specifically binds to cancer cells of epithelial origin was covalently immobilized on Au modified AAO substrate by applying streptavidin-biotin interactions. Pancreatic Cancer cells (PANC-1) were spiked into PBS buffer as well as whole blood samples without pre-labelling were captured and detected by specially designed RIfS microchip device. Our preliminary results show capability of developed device to highly selectively measure the concentration of cancer cells from 1000 to 200.000 cells/ml in buffer solution and blood samples with response time <5 minutes and volume of sample of 50 μl.
Rights: Copyright status unknown
RMID: 0020126317
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Appears in Collections:Chemical Engineering publications

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