Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/78867
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dc.contributor.author | Long, H. | - |
dc.contributor.author | Sims, D. | - |
dc.contributor.author | Heger, A. | - |
dc.contributor.author | Blackledge, N. | - |
dc.contributor.author | Kutter, C. | - |
dc.contributor.author | Wright, M. | - |
dc.contributor.author | Grutzner, F. | - |
dc.contributor.author | Odom, D. | - |
dc.contributor.author | Patient, R. | - |
dc.contributor.author | Ponting, C. | - |
dc.contributor.author | Klose, R. | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | eLife, 2013; 2013(2):e00348-1-e00348-19 | - |
dc.identifier.issn | 2050-084X | - |
dc.identifier.issn | 2050-084X | - |
dc.identifier.uri | http://hdl.handle.net/2440/78867 | - |
dc.description.abstract | Two-thirds of gene promoters in mammals are associated with regions of non-methylated DNA, called CpG islands (CGIs), which counteract the repressive effects of DNA methylation on chromatin. In cold-blooded vertebrates, computational CGI predictions often reside away from gene promoters, suggesting a major divergence in gene promoter architecture across vertebrates. By experimentally identifying non-methylated DNA in the genomes of seven diverse vertebrates, we instead reveal that non-methylated islands (NMIs) of DNA are a central feature of vertebrate gene promoters. Furthermore, NMIs are present at orthologous genes across vast evolutionary distances, revealing a surprising level of conservation in this epigenetic feature. By profiling NMIs in different tissues and developmental stages we uncover a unifying set of features that are central to the function of NMIs in vertebrates. Together these findings demonstrate an ancient logic for NMI usage at gene promoters and reveal an unprecedented level of epigenetic conservation across vertebrate evolution. | - |
dc.description.statementofresponsibility | Hannah K Long, David Sims, Andreas Heger, Neil P Blackledge, Claudia Kutter, Megan L Wright, Frank Grützner, Duncan T Odom, Roger Patient, Chris P Ponting, Robert J Klose | - |
dc.language.iso | en | - |
dc.publisher | eLife Sciences Publications Ltd | - |
dc.rights | Copyright Long et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. | - |
dc.source.uri | http://dx.doi.org/10.7554/elife.00348 | - |
dc.subject | Animals | - |
dc.subject | Xenopus | - |
dc.subject | Chickens | - |
dc.subject | Zebrafish | - |
dc.subject | Platypus | - |
dc.subject | Humans | - |
dc.subject | Mice | - |
dc.subject | Lizards | - |
dc.subject | Gene Expression Profiling | - |
dc.subject | Chromatin Assembly and Disassembly | - |
dc.subject | Species Specificity | - |
dc.subject | DNA Methylation | - |
dc.subject | Epigenesis, Genetic | - |
dc.subject | Base Sequence | - |
dc.subject | CpG Islands | - |
dc.subject | Conserved Sequence | - |
dc.subject | Databases, Genetic | - |
dc.subject | Promoter Regions, Genetic | - |
dc.subject | Biological Evolution | - |
dc.title | Epigenetic conservation at gene regulatory elements revealed by non-methylated DNA profiling in seven vertebrates | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.7554/eLife.00348#sthash.otVB3Ndl.dpuf | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Grutzner, F. [0000-0002-3088-7314] | - |
Appears in Collections: | Aurora harvest Environment Institute Leaders publications Microbiology and Immunology publications |
Files in This Item:
File | Description | Size | Format | |
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hdl_78867.pdf | Published version | 6.71 MB | Adobe PDF | View/Open |
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