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Type: Journal article
Title: Correlation of O2 uptake rate and mitochondrial activity in carrot cell cultures exposed to laminar fluid shear
Author: Wong, V.
Williams, D.
Colby, C.
Saint, D.
Citation: Biotechnology Letters, 2000; 22(24):1919-1924
Publisher: Kluwer Academic Publ
Issue Date: 2000
ISSN: 0141-5492
Abstract: Measuring uncoupled oxygen uptake rate (OUR) could provide a convenient method for quantifying changes in the metabolic activity of plant cultures caused by hydrodynamic shear. Experiments on Daucus carota (carrot) cells were performed in a novel O2-permeable Couette viscometer at varying levels of laminar shear (6 to 100 N m-2). When the uncoupled OUR of the cells was compared with mitochondrial activity (determined by 2,3,5-triphenyl tetrazolium chloride assay), a significant correlation was observed (R = 0.91 by linear regression).
DOI: 10.1023/A:1026752418909
Appears in Collections:Aurora harvest 2
Chemical Engineering publications

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