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|Title:||Correlation of O2 uptake rate and mitochondrial activity in carrot cell cultures exposed to laminar fluid shear|
|Citation:||Biotechnology Letters, 2000; 22(24):1919-1924|
|Publisher:||Kluwer Academic Publ|
|Abstract:||Measuring uncoupled oxygen uptake rate (OUR) could provide a convenient method for quantifying changes in the metabolic activity of plant cultures caused by hydrodynamic shear. Experiments on Daucus carota (carrot) cells were performed in a novel O2-permeable Couette viscometer at varying levels of laminar shear (6 to 100 N m-2). When the uncoupled OUR of the cells was compared with mitochondrial activity (determined by 2,3,5-triphenyl tetrazolium chloride assay), a significant correlation was observed (R = 0.91 by linear regression).|
|Appears in Collections:||Aurora harvest 2|
Chemical Engineering publications
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