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Type: Journal article
Title: Transposon-mediated alteration of TaMATE1B expression in wheat confers constitutive citrate efflux from root apices
Author: Tovkach, A.
Ryan, P.
Richardson, A.
Lewis, D.
Rathjen, T.
Ramesh, S.
Tyerman, S.
Delhaize, E.
Citation: Plant Physiology, 2013; 161(2):880-892
Publisher: Amer Soc Plant Physiologists
Issue Date: 2013
ISSN: 0032-0889
Statement of
Andriy Tovkach, Peter R. Ryan, Alan E. Richardson, David C. Lewis, Tina M. Rathjen, Sunita Ramesh, Stephen D. Tyerman, and Emmanuel Delhaize
Abstract: The TaMATE1B gene (for multidrug and toxic compound extrusion) from wheat (Triticum aestivum) was isolated and shown to encode a citrate transporter that is located on the plasma membrane. TaMATE1B expression in roots was induced by iron deficiency but not by phosphorus deficiency or aluminum treatment. The coding region of TaMATE1B was identical in a genotype showing citrate efflux from root apices (cv Carazinho) to one that lacked citrate efflux (cv Egret). However, sequence upstream of the coding region differed between these two genotypes in two ways. The first difference was a single-nucleotide polymorphism located approximately 2 kb upstream from the start codon in cv Egret. The second difference was an 11.1-kb transposon-like element located 25 bp upstream of the start codon in cv Carazinho that was absent from cv Egret. The influence of these polymorphisms on TaMATE1B expression was investigated using fusions to green fluorescent protein expressed in transgenic lines of rice (Oryza sativa). Fluorescence measurements in roots of rice indicated that 1.5- and 2.3-kb regions upstream of TaMATE1B in cv Carazinho (which incorporated 3′ regions of the transposon-like element) generated 20-fold greater expression in the apical 1 mm of root compared with the native promoter in cv Egret. By contrast, fluorescence in more mature tissues was similar in both cultivars. The presence of the single-nucleotide polymorphism alone consistently generated 2-fold greater fluorescence than the cv Egret promoter. We conclude that the transposon-like element in cv Carazinho extends TaMATE1B expression to the root apex, where it confers citrate efflux and enhanced aluminum tolerance.
Keywords: Cell Membrane; Plants, Genetically Modified; Triticum; Plant Roots; Aluminum; Citric Acid; Green Fluorescent Proteins; Plant Proteins; DNA Transposable Elements; Microscopy, Confocal; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, DNA; Phylogeny; Species Specificity; Gene Expression Regulation, Plant; Base Sequence; Sequence Homology, Nucleic Acid; Biological Transport; Genotype; Polymorphism, Single Nucleotide; Molecular Sequence Data; Oryza
Rights: © 2013 American Society of Plant Biologists. All Rights Reserved.
RMID: 0020124781
DOI: 10.1104/pp.112.207142
Appears in Collections:Agriculture, Food and Wine publications

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