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Type: Journal article
Title: Copper speciation and isotopic fractionation in plants: uptake and translocation mechanisms
Author: Ryan, B.
Kirby, J.
Degryse, F.
Harris, H.
McLaughlin, M.
Scheiderich, K.
Citation: New Phytologist, 2013; 199(2):367-378
Publisher: Blackwell Publishing Ltd
Issue Date: 2013
ISSN: 0028-646X
Statement of
Brooke M. Ryan, Jason K. Kirby, Fien Degryse, Hugh Harris, Mike J. McLaughlin and Kathleen Scheiderich
Abstract: THE fractionation of stable copper (Cu) isotopes during uptake into plant roots and translocation to shoots can provide information on Cu acquisition mechanisms. ISOTOPE fractionation (65Cu/63Cu) and intact tissue speciation techniques (X-ray absorption spectroscopy, XAS) were used to examine the uptake, translocation and speciation of Cu in strategy I (tomato–Solanum lycopersicum) and strategy II (oat–Avena sativa) plant species. Plants were grown in controlled solution cultures, under varied iron (Fe) conditions, to test whether the stimulation of Fe-acquiring mechanisms can affect Cu uptake in plants. ISOTOPICALLY light Cu was preferentially incorporated into tomatoes (Δ65Cuwhole plant-solution = c. −1‰), whereas oats showed minimal isotopic fractionation, with no effect of Fe supply in either species. The heavier isotope was preferentially translocated to shoots in tomato, whereas oat plants showed no significant fractionation during translocation. The majority of Cu in the roots and leaves of both species existed as sulfur-coordinated Cu(I) species resembling glutathione/cysteine-rich proteins. THE presence of isotopically light Cu in tomatoes is attributed to a reductive uptake mechanism, and the isotopic shifts within various tissues are attributed to redox cycling during translocation. The lack of isotopic discrimination in oat plants suggests that Cu uptake and translocation are not redox selective.
Keywords: Lycopersicon esculentum; Plant Leaves; Plant Roots; Copper; Iron; Isotopes; Chemical Fractionation; Biomass; Biological Transport; Fourier Analysis; Models, Biological; X-Ray Absorption Spectroscopy; Avena
Rights: © 2013 The Authors. New Phytologist © 2013 New Phytologist Trust
RMID: 0020130301
DOI: 10.1111/nph.12276
Appears in Collections:Agriculture, Food and Wine publications
Environment Institute publications

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