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Type: Journal article
Title: Characterization of ion contents and metabolic responses to salt stress of different arabidopsis AtHKT1;1 genotypes and their parental strains
Author: Hill, Camilla B.
Jha, Deepa Kumari
Bacic, Antony
Tester, Mark Alfred
Roessner, U.
Citation: Molecular Plant, 2013; 6(2):350-368
Publisher: Oxford University Press
Issue Date: 2013
ISSN: 1674-2052
School/Discipline: School of Agriculture, Food and Wine
Statement of
Camilla B. Hill, Deepa Jha, Antony Bacic, Mark Tester and Ute Roessner
Abstract: Plants employ several strategies to maintain cellular ion homeostasis under salinity stress, including mediating ion fluxes by transmembrane transport proteins and adjusting osmotic pressure by accumulating osmolytes. The HKT (high-affinity potassium transporter) gene family comprises Na+ and Na+/K+ transporters in diverse plant species, with HKT1;1 as the only member in Arabidopsis thaliana. Cell-type-specific overexpression of AtHKT1;1 has been shown to prevent shoot Na+ overaccumulation under salinity stress. Here, we analyzed a broad range of metabolites and elements in shoots and roots of different AtHKT1;1 genotypes and their parental strains before and after salinity stress, revealing a reciprocal relationship of metabolite differences between an AtHKT1;1 knockout line (hkt1;1) and the AtHKT1;1 overexpressing lines (E2586 UAS GAL4 :HKT1;1 and J2731*UAS GAL4 :HKT1;1). Although levels of root sugars were increased after salt stress in both AtHKT1;1 overexpressing lines, E2586 UAS GAL4 :HKT1;1 showed higher accumulation of the osmoprotectants trehalose, gentiobiose, and melibiose, whereas J2731*UAS GAL4 :HKT1;1 showed higher levels of sucrose and raffinose, compared with their parental lines, respectively. In contrast, the knockout line hkt1;1 showed strong increases in the levels of the tricarboxylic acid (TCA) cycle intermediates in the shoots after salt treatment. This coincided with a significant depletion of sugars, suggesting that there is an increased rate of carbon influx into the TCA cycle at a constant rate of C-efflux from the cycle, which might be needed to support plant survival during salt stress. Using correlation analysis, we identified associations between the Na+ content and several sugars, suggesting that regulation of sugar metabolism is important in plant responses to salinity stress.
Keywords: Abiotic stress; salinity; salt tolerance; HKT; sodium transporter, metabolomics; enhancer trap system
Rights: © The Author 2013. Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPB and IPPE, SIBS, CAS.
DOI: 10.1093/mp/sss125
Appears in Collections:Agriculture, Food and Wine publications

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