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dc.contributor.authorAloia, A.en
dc.contributor.authorDuffy, L.en
dc.contributor.authorPak, V.en
dc.contributor.authorLee, K.en
dc.contributor.authorSanchez-Martinez, S.en
dc.contributor.authorDerse, D.en
dc.contributor.authorHeidecker, G.en
dc.contributor.authorCornetta, K.en
dc.contributor.authorRein, A.en
dc.identifier.citationGene Therapy, 2013; 20(2):169-176en
dc.description.abstractAlthough novel retroviral vectors for use in gene-therapy products are reducing the potential for formation of replication-competent retrovirus (RCR), it remains crucial to screen products for RCR for both research and clinical purposes. For clinical-grade gammaretrovirus-based vectors, RCR screening is achieved by an extended S+L− or marker-rescue assay, whereas standard methods for replication-competent lentivirus detection are still in development. In this report, we describe a rapid and sensitive method for replication-competent gammaretrovirus detection. We used this assay to detect three members of the gammaretrovirus family and compared the sensitivity of our assay with well-established methods for retrovirus detection, including the extended S+L− assay. Results presented here demonstrate that this assay should be useful for gene-therapy product testing.en
dc.description.statementofresponsibilityAL Aloia, L Duffy, V Pak, KE Lee, S Sanchez-Martinez, D Derse, G Heidecker, K Cornetta, and A Reinen
dc.publisherNature Publishing Groupen
dc.rights© 2013 Macmillan Publishers Limiteden
dc.subjectreplication-competent gammaretrovirus; gene-therapy vectors; infectivity assayen
dc.titleA reporter system for replication-competent gammaretroviruses: the inGluc-MLV-DERSE assayen
dc.typeJournal articleen
pubs.library.collectionMolecular and Biomedical Science publicationsen
Appears in Collections:Molecular and Biomedical Science publications

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