Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
|Title:||A reporter system for replication-competent gammaretroviruses: the inGluc-MLV-DERSE assay|
|Citation:||Gene Therapy, 2013; 20(2):169-176|
|Publisher:||Nature Publishing Group|
|AL Aloia, L Duffy, V Pak, KE Lee, S Sanchez-Martinez, D Derse, G Heidecker, K Cornetta, and A Rein|
|Abstract:||Although novel retroviral vectors for use in gene-therapy products are reducing the potential for formation of replication-competent retrovirus (RCR), it remains crucial to screen products for RCR for both research and clinical purposes. For clinical-grade gammaretrovirus-based vectors, RCR screening is achieved by an extended S+L− or marker-rescue assay, whereas standard methods for replication-competent lentivirus detection are still in development. In this report, we describe a rapid and sensitive method for replication-competent gammaretrovirus detection. We used this assay to detect three members of the gammaretrovirus family and compared the sensitivity of our assay with well-established methods for retrovirus detection, including the extended S+L− assay. Results presented here demonstrate that this assay should be useful for gene-therapy product testing.|
|Keywords:||replication-competent gammaretrovirus; gene-therapy vectors; infectivity assay|
|Rights:||© 2013 Macmillan Publishers Limited|
|Appears in Collections:||Molecular and Biomedical Science publications|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.