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Type: Journal article
Title: A reporter system for replication-competent gammaretroviruses: the inGluc-MLV-DERSE assay
Author: Aloia, A.
Duffy, L.
Pak, V.
Lee, K.
Sanchez-Martinez, S.
Derse, D.
Heidecker, G.
Cornetta, K.
Rein, A.
Citation: Gene Therapy, 2013; 20(2):169-176
Publisher: Nature Publishing Group
Issue Date: 2013
ISSN: 0969-7128
Statement of
AL Aloia, L Duffy, V Pak, KE Lee, S Sanchez-Martinez, D Derse, G Heidecker, K Cornetta, and A Rein
Abstract: Although novel retroviral vectors for use in gene-therapy products are reducing the potential for formation of replication-competent retrovirus (RCR), it remains crucial to screen products for RCR for both research and clinical purposes. For clinical-grade gammaretrovirus-based vectors, RCR screening is achieved by an extended S+L− or marker-rescue assay, whereas standard methods for replication-competent lentivirus detection are still in development. In this report, we describe a rapid and sensitive method for replication-competent gammaretrovirus detection. We used this assay to detect three members of the gammaretrovirus family and compared the sensitivity of our assay with well-established methods for retrovirus detection, including the extended S+L− assay. Results presented here demonstrate that this assay should be useful for gene-therapy product testing.
Keywords: replication-competent gammaretrovirus; gene-therapy vectors; infectivity assay
Rights: © 2013 Macmillan Publishers Limited
RMID: 0020125782
DOI: 10.1038/gt.2012.18
Appears in Collections:Molecular and Biomedical Science publications

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