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Type: Journal article
Title: Tissue-specific and minor inter-individual variation in imprinting of IGF2R is a common feature of Bos taurus Concepti and not correlated with fetal weight
Author: Bebbere, D.
Bauersachs, S.
Furst, R.
Reichenbach, H.
Reichenbach, M.
Medugorac, I.
Ulbrich, S.
Wolf, E.
Ledda, S.
Hiendleder, S.
Citation: PLoS One, 2013; 8(4):1-7
Publisher: Public Library of Science
Issue Date: 2013
ISSN: 1932-6203
Statement of
Daniela Bebbere, Stefan Bauersachs, Rainer W. Fürst, Horst-Dieter Reichenbach, Myriam Reichenbach, Ivica Medugorac, Susanne E. Ulbrich, Eckhard Wolf, Sergio Ledda and Stefan Hiendleder
Abstract: The insulin-like growth factor 2 receptor (IGF2R) is essential for prenatal growth regulation and shows gene dosage effects on fetal weight that can be affected by in-vitro embryo culture. Imprinted maternal expression of murine Igf2r is well documented for all fetal tissues excluding brain, but polymorphic imprinting and biallelic expression were reported for IGF2R in human. These differences have been attributed to evolutionary changes correlated with specific reproductive strategies. However, data from species suitable for testing this hypothesis are lacking. The domestic cow (Bos taurus) carries a single conceptus with a similar gestation length as human. We identified 12 heterozygous concepti informative for imprinting studies among 68 Bos taurus fetuses at Day 80 of gestation (28% term) and found predominantly maternal IGF2R expression in all fetal tissues but brain, which escapes imprinting. Inter-individual variation in allelic expression bias, i.e. expression of the repressed paternal allele relative to the maternal allele, ranged from 4.6−8.9% in heart, 4.3−10.2% in kidney, 6.1−11.2% in liver, 4.6−15.8% in lung and 3.2−12.2% in skeletal muscle. Allelic bias for mesodermal tissues (heart, skeletal muscle) differed significantly (P<0.05) from endodermal tissues (liver, lung). The placenta showed partial imprinting with allelic bias of 22.9−34.7% and differed significantly (P<0.001) from all other tissues. Four informative fetuses were generated by in-vitro fertilization (IVF) with embryo culture and two individuals displayed fetal overgrowth. However, there was no evidence for changes in imprinting or DNA methylation after IVF, or correlations between allelic bias and fetal weight. In conclusion, imprinting of Bos taurus IGF2R is similar to mouse except in placenta, which could indicate an effect of reproductive strategy. Common minor inter-individual variation in allelic bias and absence of imprinting abnormalities in IVF fetuses suggest changes in IGF2R expression in overgrown fetuses could be modulated through other mechanisms than changes in imprinting.
Keywords: Fetus; Placenta; Animals; Cattle; Humans; Mice; Fetal Weight; Receptor, IGF Type 2; Fertilization in Vitro; Organ Specificity; Species Specificity; Gene Expression Regulation, Developmental; Genomic Imprinting; Base Sequence; Pregnancy; Alleles; Molecular Sequence Data; Female
Description: Extent: 7 p.
Rights: Copyright: © 2013 Bebbere et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
RMID: 0020126547
DOI: 10.1371/journal.pone.0059564
Appears in Collections:Medical Sciences publications

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