Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/79727
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Type: Journal article
Title: Use of insulin to increase epiblast cell number: towards a new approach for improving ESC isolation from human embryos
Author: Campbell, J.
Lane, M.
Vassiliev, I.
Nottle, M.
Citation: BioMed Research International, 2013; 2013:1-7
Publisher: Hindawi Publishing Corporation
Issue Date: 2013
ISSN: 2314-6133
2314-6141
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Responsibility: 
Jared M. Campbell, Michelle Lane, Ivan Vassiliev, and Mark B. Nottle
Abstract: Human embryos donated for embryonic stem cell (ESC) derivation have often been cryopreserved for 5–10 years. As a consequence, many of these embryos have been cultured in media now known to affect embryo viability and the number of ESC progenitor epiblast cells. Historically, these conditions supported only low levels of blastocyst development necessitating their transfer or cryopreservation at the 4–8-cell stage. As such, these embryos are donated at the cleavage stage and require further culture to the blastocyst stage before hESC derivation can be attempted. These are generally of poor quality, and, consequently, the efficiency of hESC derivation is low. Recent work using a mouse model has shown that the culture of embryos from the cleavage stage with insulin to day 6 increases the blastocyst epiblast cell number, which in turn increases the number of pluripotent cells in outgrowths following plating, and results in an increased capacity to give rise to ESCs. These findings suggest that culture with insulin may provide a strategy to improve the efficiency with which hESCs are derived from embryos donated at the cleavage stage.
Keywords: Blastocyst; Germ Layers; Animals; Humans; Mice; Insulin; Homeodomain Proteins; Culture Media; Cryopreservation; Embryo Culture Techniques; Signal Transduction; Time Factors; Octamer Transcription Factor-3; Embryonic Stem Cells; Nanog Homeobox Protein
Rights: Copyright © 2013 Jared M. Campbell et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
RMID: 0020126603
DOI: 10.1155/2013/150901
Appears in Collections:Obstetrics and Gynaecology publications

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