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Type: Journal article
Title: Effects of Osteochondrin S and select connective tissue ribonucleinate components on human osteoclasts in vitro
Author: Cantley, M.
Rainsford, K.
Haynes, D.
Citation: Journal of Pharmacy and Pharmacology, 2013; 65(8):1214-1222
Publisher: Royal Pharmaceutical Soc Great Britain
Issue Date: 2013
ISSN: 0022-3573
Statement of
Melissa D. Cantley, K. D. Rainsford and David R. Haynes
Abstract: OBJECTIVES Osteochondrin S, a natural product derived from connective tissues and yeast, is used to treat osteoarthritis. The aim of this study was to determine the effect of Osteochondrin S on human osteoclast activity in vitro. METHODS Osteoclasts were derived from human peripheral blood mononuclear cells stimulated with macrophage colony-stimulating factor and receptor activator of nuclear factor kappa B (RANK) ligand. Cells were treated with 23.5–587.2 ng/ml Osteochondrin S or 0.2–5 mg/ml of RNA components (synovia, placenta, intervertebral disc or cartilage). The effects on osteoclast formation and resorptive activity were assessed. Real-time polymerase chain reaction was conducted to assess the expression of key osteoclast genes. KEY FINDINGS Osteochondrin S and the individual RNA extracts resulted in a concentration-dependent inhibition of human osteoclast activity. Osteochondrin S did not affect RANK, nuclear factor of activated T cells (NFATc1), osteoclast-associated receptor or cathepsin K expression. However, there was a significant (P < 0.05) reduction in mRNA expression of calcitonin receptor. Osteochondrin S treatment also significantly increased the expression of osteoclast inhibitory factor interferon-β and, interestingly, increased the expression of tumour necrosis-α-like weak inducer of apoptosis (TWEAK). CONCLUSIONS Osteochondrin S inhibited the resorptive ability of osteoclasts. These actions are likely to occur at a late stage during osteoclast formation, downstream of NFATc1. Overall, the findings show that Osteochondrin S inhibition of osteoclast activity may be responsible for its beneficial effects on diseases such as osteoarthritis.
Keywords: Bone resorption; in-vitro osteoclast assay; Osteochondrin S; RNA extracts
Rights: © 2013 Royal Pharmaceutical Society, Journal of Pharmacy and Pharmacology
RMID: 0020130767
DOI: 10.1111/jphp.12088
Appears in Collections:Anatomical Sciences publications

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