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Type: Journal article
Title: Luteinizing hormone/chorionic gonadotropin bioactivity in the common marmoset (Callithrix jacchus) is due to a chorionic gonadotropin molecule with a structure intermediate between human chorionic gonadotropin and human luteinizing hormone.
Author: Simula, A.
Amato, F.
Faast, R.
Lopata, A.
Berka, J.
Norman, R.
Citation: Biology of Reproduction, 1995; 53(2):380-389
Publisher: Society for the Study of Reproduction
Issue Date: 1995
ISSN: 0006-3363
Statement of
Anthony P. Simula, Fred Amato, Renate Faast, Alexander Lopata, Jennifer Berka, and Robert J. Norman
Abstract: Chorionic gonadotropin (CG), a pregnancy-specific heterodimeric hormone found in primates, is responsible for CL rescue with pregnancy maintenance. Of the primates, the human and baboon gene sequences are the only structures so far determined. In order to study the structure and function of CG in other primates, we have isolated and sequenced the coding regions for the two subunits of marmoset CG (mCG) by the reverse transcription/polymerase chain reaction method. Study of multiple clones confirmed a high degree of homology with the human sequences (88% and 80% for the alpha and beta nucleotide sequences, respectively). Marmoset CG alpha has an extra four amino acids compared to hCG alpha, whereas the mCG beta sequence has a 3-bp deletion that maintains the reading frame and C-terminal amino acid sequence. Most of the differences between hCG beta and mCG beta peptides occur in the C-terminal region, which includes the loss of two of the O-linked glycosylation consensus sequences and the presence of an N-linked glycosylation consensus sequence. When mCG alpha and beta were co-expressed in CHO cells, assembly of biologically active hormone was confirmed by induced steroid secretion by MA10 cells. Partially purified mCG beta was used to raise anti-mCG antibodies. To date, an antibody has been obtained that is capable of detecting recombinant mCG beta, recombinant mCG dimer, and mCG dimer secreted by cultured marmoset trophoblast. Marmoset CG alpha and beta were also detectable at the transcriptional level in cultured trophoblast by in situ hybridization. This suggests that the LH/CG bioactivity reported from marmoset placentae and embryos is due to a molecule with structural features common to hLH (glycosylation pattern) and hCG (CG beta C-terminal structure).
Keywords: Cells, Cultured
CHO Cells
Macromolecular Substances
Chorionic Gonadotropin
Luteinizing Hormone
RNA-Directed DNA Polymerase
Polymerase Chain Reaction
Sequence Alignment
Gene Expression
Amino Acid Sequence
Base Sequence
Structure-Activity Relationship
Molecular Sequence Data
DOI: 10.1095/biolreprod53.2.380
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Appears in Collections:Aurora harvest
Obstetrics and Gynaecology publications

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