Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/80541
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Type: Journal article
Title: Maternal undernutrition around the time of conception and embryo number each impact on the abundance of key regulators of cardiac growth and metabolism in the fetal sheep heart
Author: Lie, S.
Sim, S.
McMillen, I.
Wyss, O.
MacLaughlin, S.
Kleemann, D.
Walker, S.
Roberts, C.
Morrison, J.
Citation: Journal of Developmental Origins of Health and Disease, 2013; 4(5):377-390
Publisher: Cambridge University Press
Issue Date: 2013
ISSN: 2040-1744
2040-1752
Statement of
Responsibility: 
S. Lie, S. M. Sim, I. C. McMillen, O. Williams-Wyss, S. M. MacLaughlin, D. O. Kleemann, S. K. Walker, C. T. Roberts and J. L. Morrison
Abstract: Poor maternal nutrition before and during pregnancy is associated with an increased risk of cardiovascular disease in later life. To determine the impact of maternal undernutrition during the periconceptional (PCUN: -45 days to 6 days) and preimplantation (PIUN: 0-6 days) periods on cardiac growth and metabolism, we have quantified the mRNA and protein abundance of key regulators of cardiac growth and metabolism in the left ventricle of the sheep fetus in late gestation. The cardiac protein abundance of AMP-activated protein kinase (AMPK), phospho-acetyl CoA carboxykinase (ACC) and pyruvate dehydrogenase kinase-4 (PDK-4) were decreased, whereas ACC was increased in singletons in the PCUN and PIUN groups. In twins, however, cardiac ACC was decreased in the PCUN and PIUN groups, and carnitine palmitoyltransferase-1 (CPT-1) was increased in the PIUN group. In singletons, the cardiac abundance of insulin receptor β (IRβ) was decreased in the PCUN group, and phosphoinositide-dependent protein kinase-1 (PDPK-1) was decreased in the PCUN and PIUN groups. In twins, however, the cardiac abundance of IRβ and phospho-Akt substrate 160kDa (pAS160) were increased in the PIUN group. The cardiac abundance of insulin-like growth factor-2 receptor (IGF-2R), protein kinase C alpha (PKCα) and mammalian target of rapamycin (mTOR) were decreased in PCUN and PIUN singletons and extracellular-signal-regulated kinase (ERK) was also decreased in the PIUN singletons. In contrast, in twins, cardiac abundance of IGF-2R and PKCα were increased in the PCUN and PIUN groups, phospho-ribosomal protein S6 (pRPS6) was increased in the PCUN group, and ERK and eukaryotic initiation factor 4E (eIF4E) were also increased in the PIUN fetuses. In conclusion, maternal undernutrition limited to around the time of conception is sufficient to alter the abundance of key factors regulating cardiac growth and metabolism and this may increase the propensity for cardiovascular diseases in later life.
Keywords: embryo; heart; hypertrophy; nutrition; periconceptional; pregnancy
Rights: © Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2013
RMID: 0020131308
DOI: 10.1017/S2040174413000354
Grant ID: http://purl.org/au-research/grants/nhmrc/1020749
Appears in Collections:Obstetrics and Gynaecology publications

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