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Type: Journal article
Title: Cytoplasmic organelle DNA preferentially inserts into open chromatin
Author: Wang, D.
Timmis, J.
Citation: Genome Biology and Evolution, 2013; 5(6):1060-1064
Publisher: Oxford University Press
Issue Date: 2013
ISSN: 1759-6653
Statement of
Dong Wang and Jeremy N. Timmis
Abstract: DNA transfer from chloroplasts and mitochondria to the nucleus is ongoing in eukaryotes but the mechanisms involved are poorly understood. Mitochondrial DNA was observed to integrate into the nuclear genome through DNA double-strand break repair in Nicotiana tabacum. Here, 14 nuclear insertions of chloroplast DNA (nupts) that are unique to Oryza sativa subsp. indica were identified. Comparisons with the preinsertion nuclear loci identified in the related subspecies, O. sativa subsp. japonica, which lacked these nupts, indicated that chloroplast DNA had integrated by nonhomologous end joining. Analyzing public DNase-seq data revealed that nupts were significantly more frequent in open chromatin regions of the nucleus. This preference was tested further in the chimpanzee genome by comparing nuclear loci containing integrants of mitochondrial DNA (numts) with their corresponding numt-lacking preinsertion sites in the human genome. Mitochondrial DNAs also tended to insert more frequently into regions of open chromatin revealed by human DNase-seq and Formaldehyde-Assisted Isolation of Regulatory Elements-seq databases
Keywords: Endosymbiotic gene transfer
double-strand break repair
open chromatin
Rights: © The Author(s) 2013. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution
DOI: 10.1093/gbe/evt070
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Appears in Collections:Aurora harvest
Molecular and Biomedical Science publications

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