Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/80621
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Appleby, S. | - |
dc.contributor.author | Jessup, C. | - |
dc.contributor.author | Mortimer, L. | - |
dc.contributor.author | Kirk, K. | - |
dc.contributor.author | Brereton, H. | - |
dc.contributor.author | Coster, D. | - |
dc.contributor.author | Tan, C. | - |
dc.contributor.author | Williams, K. | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | British Journal of Ophthalmology, 2013; 97(1):101-105 | - |
dc.identifier.issn | 0007-1161 | - |
dc.identifier.issn | 1468-2079 | - |
dc.identifier.uri | http://hdl.handle.net/2440/80621 | - |
dc.description.abstract | <h4>Aim</h4>To investigate whether expression of an anti-CD4 antibody fragment (scFv) by a lentivector-transduced donor cornea can prolong rat corneal allograft survival.<h4>Methods</h4>Inbred Fischer 344 rats received penetrating corneal allografts from Wistar-Furth donors after a 3 h transduction of the donor cornea with a lentivector carrying anti-CD4scFv cDNA (Lv-CD4scFv), a lentivector carrying the reporter gene-enhanced yellow fluorescence protein (LV-eYFP), or an adenoviral vector carrying anti-CD4 scFv cDNA (Ad-CD4scFv). Unmodified controls were also performed. Graft survival was assessed by corneal clarity, and rejection was confirmed histologically.<h4>Results</h4>In organ-cultured corneas, expression of anti-CD4 scFv was detected at 2 days post-transduction with the adenoviral vector, compared with 5 days post-transduction with the lentivector, and was 10-fold higher than the former. More inflammation was observed in Ad-CD4scFv-modified allografts than in Lv-CD4scFv-modified grafts at 15 days postsurgery (p=0.01). The median time to rejection for unmodified, LV-eYFP and Ad-CD4scFv grafts was day 17, compared with day 22 for Lv-CD4scFv grafts (p≤0.018).<h4>Conclusion</h4>Donor corneas transduced with a lentiviral vector carrying anti-CD4scFv cDNA showed a modest but significant prolongation in graft survival compared with unmodified, Lv-eYFP and Ad-CD4scFv grafts. However, rejection still occurred in all Lv-CD4scFv grafts, indicating that sensitisation may have been delayed but was not prevented. | - |
dc.description.statementofresponsibility | Sarah Louise Appleby, Claire F Jessup, Lauren A Mortimer, Kirsty Kirk, Helen M Brereton, Douglas John Coster, Chuan K Tan, Keryn A Williams | - |
dc.language.iso | en | - |
dc.publisher | British Med Journal Publ Group | - |
dc.rights | Copyright status unknown | - |
dc.source.uri | http://dx.doi.org/10.1136/bjophthalmol-2012-302360 | - |
dc.subject | Cornea | - |
dc.subject | CD4-Positive T-Lymphocytes | - |
dc.subject | Animals | - |
dc.subject | Rats, Inbred F344 | - |
dc.subject | Rats, Inbred WF | - |
dc.subject | Rats | - |
dc.subject | Adenoviridae | - |
dc.subject | Bacterial Proteins | - |
dc.subject | Luminescent Proteins | - |
dc.subject | Fluorescent Dyes | - |
dc.subject | Enzyme-Linked Immunosorbent Assay | - |
dc.subject | Keratoplasty, Penetrating | - |
dc.subject | Transplantation, Homologous | - |
dc.subject | Flow Cytometry | - |
dc.subject | Transfection | - |
dc.subject | Graft Survival | - |
dc.subject | Gene Expression Regulation | - |
dc.subject | Genes, Reporter | - |
dc.subject | Genetic Vectors | - |
dc.subject | Tissue Donors | - |
dc.subject | Male | - |
dc.subject | Single-Chain Antibodies | - |
dc.title | Expression of an anti-CD4 single-chain antibody fragment from the donor cornea can prolong corneal allograft survival in inbred rats | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1136/bjophthalmol-2012-302360 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Jessup, C. [0000-0003-1184-6653] | - |
Appears in Collections: | Aurora harvest Paediatrics publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.