Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/80621
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dc.contributor.authorAppleby, S.-
dc.contributor.authorJessup, C.-
dc.contributor.authorMortimer, L.-
dc.contributor.authorKirk, K.-
dc.contributor.authorBrereton, H.-
dc.contributor.authorCoster, D.-
dc.contributor.authorTan, C.-
dc.contributor.authorWilliams, K.-
dc.date.issued2013-
dc.identifier.citationBritish Journal of Ophthalmology, 2013; 97(1):101-105-
dc.identifier.issn0007-1161-
dc.identifier.issn1468-2079-
dc.identifier.urihttp://hdl.handle.net/2440/80621-
dc.description.abstract<h4>Aim</h4>To investigate whether expression of an anti-CD4 antibody fragment (scFv) by a lentivector-transduced donor cornea can prolong rat corneal allograft survival.<h4>Methods</h4>Inbred Fischer 344 rats received penetrating corneal allografts from Wistar-Furth donors after a 3 h transduction of the donor cornea with a lentivector carrying anti-CD4scFv cDNA (Lv-CD4scFv), a lentivector carrying the reporter gene-enhanced yellow fluorescence protein (LV-eYFP), or an adenoviral vector carrying anti-CD4 scFv cDNA (Ad-CD4scFv). Unmodified controls were also performed. Graft survival was assessed by corneal clarity, and rejection was confirmed histologically.<h4>Results</h4>In organ-cultured corneas, expression of anti-CD4 scFv was detected at 2 days post-transduction with the adenoviral vector, compared with 5 days post-transduction with the lentivector, and was 10-fold higher than the former. More inflammation was observed in Ad-CD4scFv-modified allografts than in Lv-CD4scFv-modified grafts at 15 days postsurgery (p=0.01). The median time to rejection for unmodified, LV-eYFP and Ad-CD4scFv grafts was day 17, compared with day 22 for Lv-CD4scFv grafts (p≤0.018).<h4>Conclusion</h4>Donor corneas transduced with a lentiviral vector carrying anti-CD4scFv cDNA showed a modest but significant prolongation in graft survival compared with unmodified, Lv-eYFP and Ad-CD4scFv grafts. However, rejection still occurred in all Lv-CD4scFv grafts, indicating that sensitisation may have been delayed but was not prevented.-
dc.description.statementofresponsibilitySarah Louise Appleby, Claire F Jessup, Lauren A Mortimer, Kirsty Kirk, Helen M Brereton, Douglas John Coster, Chuan K Tan, Keryn A Williams-
dc.language.isoen-
dc.publisherBritish Med Journal Publ Group-
dc.rightsCopyright status unknown-
dc.source.urihttp://dx.doi.org/10.1136/bjophthalmol-2012-302360-
dc.subjectCornea-
dc.subjectCD4-Positive T-Lymphocytes-
dc.subjectAnimals-
dc.subjectRats, Inbred F344-
dc.subjectRats, Inbred WF-
dc.subjectRats-
dc.subjectAdenoviridae-
dc.subjectBacterial Proteins-
dc.subjectLuminescent Proteins-
dc.subjectFluorescent Dyes-
dc.subjectEnzyme-Linked Immunosorbent Assay-
dc.subjectKeratoplasty, Penetrating-
dc.subjectTransplantation, Homologous-
dc.subjectFlow Cytometry-
dc.subjectTransfection-
dc.subjectGraft Survival-
dc.subjectGene Expression Regulation-
dc.subjectGenes, Reporter-
dc.subjectGenetic Vectors-
dc.subjectTissue Donors-
dc.subjectMale-
dc.subjectSingle-Chain Antibodies-
dc.titleExpression of an anti-CD4 single-chain antibody fragment from the donor cornea can prolong corneal allograft survival in inbred rats-
dc.typeJournal article-
dc.identifier.doi10.1136/bjophthalmol-2012-302360-
pubs.publication-statusPublished-
dc.identifier.orcidJessup, C. [0000-0003-1184-6653]-
Appears in Collections:Aurora harvest
Paediatrics publications

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