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Type: Journal article
Title: Mesoporous silica nanoparticles act as a self-adjuvant for ovalbumin model antigen in mice
Author: Mahony, D.
Cavallaro, A.
Stahr, F.
Mahony, T.
Qiao, S.
Mitter, N.
Citation: Small, 2013; 9(18):3138-3146
Publisher: Wiley - VCH Verlag GmbH & Co KGaA
Issue Date: 2013
ISSN: 1613-6810
Statement of
Donna Mahony, Antonino S. Cavallaro, Frances Stahr, Timothy J. Mahony, Shi Zhang Qiao, Neena Mitter
Abstract: Immunization to the model protein antigen ovalbumin (OVA) is investigated using MCM-41 mesoporous silica nanoparticles as a novel vaccine delivery vehicle and adjuvant system in mice. The effects of amino surface functionalization and adsorption time on OVA adsorption to nanoparticles are assessed. Amino-functionalized MCM-41 (AM-41) shows an effect on the amount of OVA binding, with 2.5-fold increase in binding capacity (72 mg OVA/g AM-41) compared to nonfunctionalized MCM-41 (29 mg OVA/g MCM-41). Immunization studies in mice with a 10 μg dose of OVA adsorbed to AM-41 elicits both antibody and cell-mediated immune responses following three subcutaneous injections. Immunizations at a lower 2 μg dose of OVA adsorbed to AM-41 particles results in an antibody response but not cell-mediated immunity. The level of antibody responses following immunization with nanoformulations containing either 2 μg or 10 μg of OVA are only slightly lower than that in mice which receive 50 μg OVA adjuvanted with QuilA, a crude mixture of saponins extracted from the bark of the Quillaja saponaria Molina tree. This is a significant result, since it demonstrates that AM-41 nanoparticles are self-adjuvanting and elicit immune responses at reduced antigen doses in vivo compared to a conventional delivery system. Importantly, there are no local or systemic negative effects in animals injected with AM-41. Histopathological studies of a range of tissue organs show no changes in histopathology of the animals receiving nanoparticles over a six week period. These results establish the biocompatible MCM-41 silica nanoparticles as a new method for vaccine delivery which incorporates a self-adjuvant effect.
Keywords: adjuvants
mesoporous silica nanoparticles
drug delivery
Rights: Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
DOI: 10.1002/smll.201300012
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Chemical Engineering publications

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