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Type: Journal article
Title: False-positive TUNEL staining observed in SV40 based transgenic murine prostate cancer models
Author: Lawrence, M.
Blyth, B.
Ormsby, R.
Tilley, W.
Sykes, P.
Citation: Transgenic Research, 2013; 22(5):1037-1047
Publisher: Kluwer Academic Publ
Issue Date: 2013
ISSN: 0962-8819
Statement of
M. D. Lawrence, B. J. Blyth, R. J. Ormsby, W. D. Tilley, P. J. Sykes
Abstract: The TRAMP (Transgenic Adenocarcinoma of the Mouse Prostate) and LADY (Probasinlarge T antigen transgenic mouse) mice are widely used autochthonous models of prostate cancer. Both models utilise probasin promoters to direct androgenregulated expression of oncogenic SV40 specifically to epithelial cells of the mouse prostate. The oncogenic processes and phenotypes which result mimic many features of human prostate cancer, making these transgenic mouse models useful experimental systems. The terminal deoxynucleotidyl transferase (Tdt) -mediated dUTP in situ nick end labelling (TUNEL) assay is a commonly used method for the detection of cells undergoing apoptosis. In this study, we demonstrate false-positive TUNEL staining in frozen prostate tissue from TRAMP and LADY mice, which was not observed in non-transgenic control animals and is not due to non-specific binding of labelled-dUTP substrate. The false-positive signal co-localised with large SV40 T-antigen expression. False-positive signal was apparent using multiple commercial TUNEL kits with different detection systems. These results caution against the use of the TUNEL assay for detection of apoptosis in frozen prostate tissue of large T-antigen based autochthonous transgenic models of prostate cancer
Keywords: TRAMP; LADY; Prostate; TUNEL; Apoptosis; SV40; TAg
Rights: © Springer Science+Business Media Dordrecht 2013
RMID: 0020131795
DOI: 10.1007/s11248-013-9694-7
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Appears in Collections:Medicine publications

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