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dc.contributor.authorOng, C.en
dc.contributor.authorPotter, A.en
dc.contributor.authorTrappetti, C.en
dc.contributor.authorWalker, M.en
dc.contributor.authorJennings, M.en
dc.contributor.authorPaton, J.en
dc.contributor.authorMcEwan, A.en
dc.identifier.citationInfection and Immunity, 2013; 81(2):421-429en
dc.description.abstractStreptococcus pneumoniae (the pneumococcus) is a major human pathogen that is carried asymptomatically in the nasopharynx by up to 70% of the human population. Translocation of the bacteria into internal sites can cause a range of diseases, such as pneumonia, otitis media, meningitis, and bacteremia. This transition from nasopharynx to growth at systemic sites means that the pneumococcus needs to adjust to a variety of environmental conditions, including transition metal ion availability. Although it is an important nutrient, iron potentiates oxidative stress, and it is established that in S. pneumoniae, expression of iron transport systems and proteins that protect against oxidative stress are regulated by an orphan response regulator, RitR. In this study, we investigated the effect of iron and manganese ion availability on the growth of a ritR mutant. Deletion of ritR led to impaired growth of bacteria in high-iron medium, but this phenotype could be suppressed with the addition of manganese. Measurement of metal ion accumulation indicated that manganese prevents iron accumulation. Furthermore, the addition of manganese also led to a reduction in the amount of hydrogen peroxide produced by bacterial cells. Studies of virulence in a murine model of infection indicated that RitR was not essential for pneumococcal survival and suggested that derepression of iron uptake systems may enhance the survival of pneumococci in some niches.en
dc.description.statementofresponsibilityCheryl-Lynn Y. Ong, Adam J. Potter, Claudia Trappetti, Mark J. Walker, Michael P. Jennings, James C. Paton, Alastair G. McEwanen
dc.publisherAmer Soc Microbiologyen
dc.rightsCopyright © 2013, American Society for Microbiology. All Rights Reserved.en
dc.subjectAnimals; Mice; Streptococcus pneumoniae; Pneumococcal Infections; Hydrogen Peroxide; Iron; Manganese; Bacterial Proteins; Cation Transport Proteins; Virulence; Gene Expression Regulation, Bacterial; Ion Transport; Oxidative Stress; Mutation; Femaleen
dc.titleInterplay between manganese and iron in pneumococcal pathogenesis: Role of the orphan response regulator ritRen
dc.typeJournal articleen
pubs.library.collectionMolecular and Biomedical Science publicationsen
dc.identifier.orcidTrappetti, C. [0000-0001-8272-0068]en
dc.identifier.orcidPaton, J. [0000-0001-9807-5278]en
Appears in Collections:Molecular and Biomedical Science publications

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