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Type: Journal article
Title: One-step cloning and chromosomal integration of DNA
Author: St-Pierre, F.
Cui, L.
Priest, D.
Endy, D.
Dodd, I.
Shearwin, K.
Citation: ACS Synthetic Biology, 2013; 2(9):537-541
Publisher: American Chemical Society
Issue Date: 2013
ISSN: 2161-5063
Statement of
François St-Pierre, Lun Cui, David G. Priest, Drew Endy, Ian B. Dodd, and Keith E. Shearwin
Abstract: We describe "clonetegration", a method for integrating DNA into prokaryotic chromosomes that approaches the simplicity of cloning DNA within extrachromosomal vectors. Compared to existing techniques, clonetegration drastically decreases the time and effort needed for integration of single or multiple DNA fragments. Additionally, clonetegration facilitates cloning and expression of genetic elements that are impossible to propagate within typical multicopy plasmids.
Keywords: Chromosomes, Bacterial; Escherichia coli; Salmonella typhimurium; DNA; DNA Primers; Cloning, Molecular; Genetic Engineering; Transformation, Genetic; Genetic Vectors
Rights: Copyright © 2013 American Chemical Society
RMID: 0020131949
DOI: 10.1021/sb400021j
Grant ID:
Appears in Collections:Molecular and Biomedical Science publications

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