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Type: Journal article
Title: Duck hepatitis B virus covalently closed circular DNA appears to survive hepatocyte mitosis in the growing liver
Author: Reaiche-Miller, G.
Thorpe, M.
Low, H.
Qiao, Q.
Scougall, C.
Mason, W.
Litwin, S.
Jilbert, A.
Citation: Virology, 2013; 446(1-2):357-364
Publisher: Academic Press Inc
Issue Date: 2013
ISSN: 0042-6822
Statement of
Georget Y. Reaiche-Miller, Michael Thorpe, Huey Chi Low, Qiao Qiao, Catherine A. Scougall, William S. Mason, Samuel Litwin, Allison R. Jilbert
Abstract: Nucleos(t)ide analogues that inhibit hepatitis B virus (HBV) DNA replication are typically used as monotherapy for chronically infected patients. Treatment with a nucleos(t)ide analogue eliminates most HBV DNA replication intermediates and produces a gradual decline in levels of covalently closed circular DNA (cccDNA), the template for viral RNA synthesis. It remains uncertain if levels of cccDNA decline primarily through hepatocyte death, or if loss also occurs during hepatocyte mitosis. To determine if cccDNA survives mitosis, growing ducklings infected with duck hepatitis B virus (DHBV) were treated with the nucleoside analogue, Entecavir. Viremia was suppressed at least 10(5)-fold, during a period when average liver mass increased 23-fold. Analysis of the data suggested that if cccDNA synthesis was completely inhibited, at least 49% of cccDNA survived hepatocyte mitosis. However, there was a large duck-to-duck variation in cccDNA levels, suggesting that low level cccDNA synthesis may contribute to this apparent survival through mitosis.
Keywords: Hepatitis B virus (HBV)
Duck hepatitis B virus (DHBV)
Covalently closed circular DNA (cccDNA)
Hepatocyte mitosis
Nucleoside analogue
Entecavir (ETV)
Survival or loss of cccDNA
Rights: Crown copyright © 2013
DOI: 10.1016/j.virol.2013.08.014
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Appears in Collections:Aurora harvest 4
Molecular and Biomedical Science publications

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