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|Title:||Challenges of 'sticky' co-immunoprecipitation: Polyalanine tract protein-protein interactions|
|Citation:||Tandem repeats in genes, proteins, and disease : methods and protocols, 2013 / Hatters, D., Hannan, A. (ed./s), Ch.9, pp.121-133|
|Series/Report no.:||Methods in Molecular Biology ; 1017|
|T. R. Mattiske, May H. Tan, Jozef Gécz, and Cheryl Shoubridge|
|Abstract:||Co-immunoprecipitation (Co-IP) (followed by immunoblotting) is a technique widely used to characterize specific protein–protein interactions. Investigating interactions of proteins containing “sticky” polyalanine (PolyA) tracts encounters difficulties using conventional Co-IP procedures. Here, we present strategies to specifically capture proteins containing these difficult PolyA tracts, enabling subsequent robust detection of interacting proteins by Co-IP.|
|Keywords:||Polyalanine tract; ARX; Co-immunoprecipitation; Protein–protein interaction|
|Description:||ISSN 1064-3745 (print) ISSN 1940-6029 (electronic)|
|Rights:||© Springer Science+Business Media New York 2013|
|Appears in Collections:||Paediatrics publications|
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