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Type: Book chapter
Title: Challenges of 'sticky' co-immunoprecipitation: Polyalanine tract protein-protein interactions
Author: Mattiske, T.
Tan, M.
Gecz, J.
Shoubridge, C.
Citation: Tandem repeats in genes, proteins, and disease : methods and protocols, 2013 / Hatters, D., Hannan, A. (ed./s), vol.1017, Ch.9, pp.121-133
Publisher: Springer
Issue Date: 2013
Series/Report no.: Methods in Molecular Biology ; 1017
ISBN: 9781627034371
Editor: Hatters, D.
Hannan, A.
Statement of
T. R. Mattiske, May H. Tan, Jozef Gécz, and Cheryl Shoubridge
Abstract: Co-immunoprecipitation (Co-IP) (followed by immunoblotting) is a technique widely used to characterize specific protein–protein interactions. Investigating interactions of proteins containing “sticky” polyalanine (PolyA) tracts encounters difficulties using conventional Co-IP procedures. Here, we present strategies to specifically capture proteins containing these difficult PolyA tracts, enabling subsequent robust detection of interacting proteins by Co-IP.
Keywords: Polyalanine tract; ARX; Co-immunoprecipitation; Protein–protein interaction
Description: ISSN 1064-3745 (print) ISSN 1940-6029 (electronic)
Rights: © Springer Science+Business Media New York 2013
DOI: 10.1007/978-1-62703-438-8_9
Appears in Collections:Aurora harvest 4
Paediatrics publications

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