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https://hdl.handle.net/2440/81224
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Type: | Book chapter |
Title: | Challenges of 'sticky' co-immunoprecipitation: Polyalanine tract protein-protein interactions |
Author: | Mattiske, T. Tan, M. Gecz, J. Shoubridge, C. |
Citation: | Tandem repeats in genes, proteins, and disease : methods and protocols, 2013 / Hatters, D., Hannan, A. (ed./s), vol.1017, Ch.9, pp.121-133 |
Publisher: | Springer |
Issue Date: | 2013 |
Series/Report no.: | Methods in Molecular Biology ; 1017 |
ISBN: | 9781627034371 |
Editor: | Hatters, D. Hannan, A. |
Statement of Responsibility: | T. R. Mattiske, May H. Tan, Jozef Gécz, and Cheryl Shoubridge |
Abstract: | Co-immunoprecipitation (Co-IP) (followed by immunoblotting) is a technique widely used to characterize specific protein–protein interactions. Investigating interactions of proteins containing “sticky” polyalanine (PolyA) tracts encounters difficulties using conventional Co-IP procedures. Here, we present strategies to specifically capture proteins containing these difficult PolyA tracts, enabling subsequent robust detection of interacting proteins by Co-IP. |
Keywords: | Polyalanine tract; ARX; Co-immunoprecipitation; Protein–protein interaction |
Description: | ISSN 1064-3745 (print) ISSN 1940-6029 (electronic) |
Rights: | © Springer Science+Business Media New York 2013 |
DOI: | 10.1007/978-1-62703-438-8_9 |
Appears in Collections: | Aurora harvest 4 Paediatrics publications |
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