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https://hdl.handle.net/2440/81224
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dc.contributor.author | Mattiske, T. | - |
dc.contributor.author | Tan, M. | - |
dc.contributor.author | Gecz, J. | - |
dc.contributor.author | Shoubridge, C. | - |
dc.contributor.editor | Hatters, D. | - |
dc.contributor.editor | Hannan, A. | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Tandem repeats in genes, proteins, and disease : methods and protocols, 2013 / Hatters, D., Hannan, A. (ed./s), vol.1017, Ch.9, pp.121-133 | - |
dc.identifier.isbn | 9781627034371 | - |
dc.identifier.uri | http://hdl.handle.net/2440/81224 | - |
dc.description | ISSN 1064-3745 (print) ISSN 1940-6029 (electronic) | - |
dc.description.abstract | Co-immunoprecipitation (Co-IP) (followed by immunoblotting) is a technique widely used to characterize specific protein–protein interactions. Investigating interactions of proteins containing “sticky” polyalanine (PolyA) tracts encounters difficulties using conventional Co-IP procedures. Here, we present strategies to specifically capture proteins containing these difficult PolyA tracts, enabling subsequent robust detection of interacting proteins by Co-IP. | - |
dc.description.statementofresponsibility | T. R. Mattiske, May H. Tan, Jozef Gécz, and Cheryl Shoubridge | - |
dc.language.iso | en | - |
dc.publisher | Springer | - |
dc.relation.ispartofseries | Methods in Molecular Biology ; 1017 | - |
dc.rights | © Springer Science+Business Media New York 2013 | - |
dc.subject | Polyalanine tract; ARX; Co-immunoprecipitation; Protein–protein interaction | - |
dc.title | Challenges of 'sticky' co-immunoprecipitation: Polyalanine tract protein-protein interactions | - |
dc.type | Book chapter | - |
dc.identifier.doi | 10.1007/978-1-62703-438-8_9 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Mattiske, T. [0000-0002-9581-5370] | - |
dc.identifier.orcid | Gecz, J. [0000-0002-7884-6861] | - |
dc.identifier.orcid | Shoubridge, C. [0000-0002-0157-3084] | - |
Appears in Collections: | Aurora harvest 4 Paediatrics publications |
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