Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/81517
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Type: Book chapter
Title: PCR amplification and sequence analysis of GC-rich sequences: Aristaless-related homeobox example
Author: Tan, M.
Gecz, J.
Shoubridge, C.
Citation: Tandem repeats in genes, proteins, and disease : methods and protocols, 2013 / Hatters, D., Hannan, A. (ed./s), Ch.8, pp.105-120
Publisher: Springer
Publisher Place: United States
Issue Date: 2013
Series/Report no.: Methods in Molecular Biology; vol. 1017
ISBN: 9781627034371
Statement of
Responsibility: 
May H. Tan , Jozef Gécz , and Cheryl Shoubridge
Abstract: PCR ampli fi cation (followed by mutation scanning or direct sequencing) is a technique widely used in mutation detection and molecular studies of disease-causing genes, such as ARX . PCR ampli fi cation of high GC-rich regions encounters dif fi culties using conventional PCR procedures. Here, we present the strategies to amplify and sequence these GC-rich regions for the purposes of mutation screening and other molecular analyses.
Keywords: Polyalanine tracts; GC-rich; PCR; Sequencing; ARX
Description: ISSN 1064-3745 (print) ISSN 1940-6029 (electronic)
Rights: © Springer Science+Business Media New York 2013
RMID: 0020131327
DOI: 10.1007/978-1-62703-438-8_8
Appears in Collections:Paediatrics publications

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