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|Title:||PCR amplification and sequence analysis of GC-rich sequences: Aristaless-related homeobox example|
|Citation:||Tandem repeats in genes, proteins, and disease : methods and protocols, 2013 / Hatters, D., Hannan, A. (ed./s), Ch.8, pp.105-120|
|Publisher Place:||United States|
|Series/Report no.:||Methods in Molecular Biology; vol. 1017|
|May H. Tan , Jozef Gécz , and Cheryl Shoubridge|
|Abstract:||PCR ampli fi cation (followed by mutation scanning or direct sequencing) is a technique widely used in mutation detection and molecular studies of disease-causing genes, such as ARX . PCR ampli fi cation of high GC-rich regions encounters dif fi culties using conventional PCR procedures. Here, we present the strategies to amplify and sequence these GC-rich regions for the purposes of mutation screening and other molecular analyses.|
|Keywords:||Polyalanine tracts; GC-rich; PCR; Sequencing; ARX|
|Description:||ISSN 1064-3745 (print) ISSN 1940-6029 (electronic)|
|Rights:||© Springer Science+Business Media New York 2013|
|Appears in Collections:||Paediatrics publications|
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