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https://hdl.handle.net/2440/81536
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Type: | Journal article |
Title: | Proteomic analysis during capsicum ripening reveals differential expression of ACC oxidase isoform 4 and other candidates |
Author: | Wan Kamaruddin, W. Able, J. Stangoulis, J. Able, A. |
Citation: | Functional Plant Biology: an international journal of plant function, 2013; 40(11):1115-1128 |
Publisher: | C S I R O Publishing |
Issue Date: | 2013 |
ISSN: | 1445-4408 1445-4416 |
Statement of Responsibility: | Wan M. Aizat, Jason A. Able, James C. R. Stangoulis and Amanda J. Able |
Abstract: | Capsicum (Capsicum annuum L.) is categorised as a non-climacteric fruit that exhibits limited ethylene production during ripening and the molecular mechanisms associated with this process are poorly understood. A proteomic approach was used to identify the differentially expressed proteins during various ripening stages (Green (G), Breaker Red 1 (BR1) and Light Red (LR)) and the genes associated with their synthesis. From 2D gel electrophoresis (2DGE), seven protein spots were identified as selectively present either in G or BR1 and are involved in carbon metabolism, colour and fruit development, protein synthesis and chaperones or biosynthesis of amino acids and polyamines. One candidate of interest, 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO) is known to be involved in ethylene biosynthesis and was only present in BR1 and is related to the tomato ACO isoform 4 (LeACO4) and hence named CaACO4. CaACO4 RNA expression as well as total ACO protein expression in multiple stages of ripening (G, Breaker (B), BR1, Breaker Red 2 (BR2), LR and Deep Red (DR)) corresponded to the 2DGE protein spot abundance in breaker stages. Our findings highlight the involvement of the ethylene pathway in non-climacteric fruit ripening. |
Keywords: | Capsicum annuum, fruit ripening, non-climacteric ripening, pepper, proteomics, 2D gel electrophoresis |
Rights: | Copyright status unknown |
DOI: | 10.1071/FP12330 |
Published version: | http://dx.doi.org/10.1071/fp12330 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 4 |
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