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dc.contributor.authorWard, H.-
dc.contributor.authorHiggs, N.-
dc.contributor.authorBlackmore, T.-
dc.contributor.authorSadlon, T.-
dc.identifier.citationImmunology and Cell Biology, 1997; 75(5):508-510-
dc.description.abstractThe 5' flanking region of human factor H was cloned using nested polymerase chain reaction (PCR) and the promoter finder method. A total of 1.2 kb has been sequenced and a number of putative regulatory elements identified including glucocorticoid response elements, cAMP responsive element, HTF-1, and acute phase signal sequences. A 717 b.p. fragment was cloned into a CAT reporter vector and transfected into HeLa cells. A series of truncations from the 5' end of this fragment were also cloned into the CAT vector. Analysis of CAT activity of the cell lysates showed that the region from -699 to +18 is likely to contain promoter elements for the factor H gene as it was able to drive transcription of the CAT gene.-
dc.description.statementofresponsibilityHelena M Ward, Naomi H Higgs, Timothy K Blackmore, Tania A Sadlon and David L Gordon-
dc.publisherNature Publishing Group-
dc.rightsCopyright status unknown-
dc.subjectGene promoter-
dc.titleCloning and analysis of the human complement factor H gene promoter-
dc.typeJournal article-
dc.identifier.orcidWard, H. [0000-0002-3831-1205]-
Appears in Collections:Aurora harvest 4
Medical Education Unit publications

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