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Type: Journal article
Title: Non-invasive airway health assessment: Synchrotron imaging reveals effects of rehydrating treatments on mucociliary transit in-vivo
Author: Donnelley, M.
Morgan, K.
Siu, K.
Farrow, N.
Stahr, C.
Boucher, R.
Fouras, A.
Parsons, D.
Citation: Scientific Reports, 2014; 4(3689):1-7
Publisher: Nature Publishing Group
Issue Date: 2014
ISSN: 2045-2322
Statement of
Martin Donnelley, Kaye S. Morgan, Karen K. W. Siu, Nigel R. Farrow, Charlene S. Stahr, Richard C. Boucher, Andreas Fouras & David W. Parsons
Abstract: To determine the efficacy of potential cystic fibrosis (CF) therapies we have developed a novel mucociliary transit (MCT) measurement that uses synchrotron phase contrast X-ray imaging (PCXI) to non-invasively measure the transit rate of individual micron-sized particles deposited into the airways of live mice. The aim of this study was to image changes in MCT produced by a rehydrating treatment based on hypertonic saline (HS), a current CF clinical treatment. Live mice received HS containing a long acting epithelial sodium channel blocker (P308); isotonic saline; or no treatment, using a nebuliser integrated within a small-animal ventilator circuit. Marker particle motion was tracked for 20 minutes using PCXI. There were statistically significant increases in MCT in the isotonic and HS-P308 groups. The ability to quantify in vivo changes in MCT may have utility in pre-clinical research studies designed to bring new genetic and pharmaceutical treatments for respiratory diseases into clinical trials.
Keywords: Biomedical engineering
Pre-clinical studies
Rights: This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit © The Authors
DOI: 10.1038/srep03689
Appears in Collections:Aurora harvest 4
Paediatrics publications

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