Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/81839
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Type: | Journal article |
Title: | Non-invasive airway health assessment: Synchrotron imaging reveals effects of rehydrating treatments on mucociliary transit in-vivo |
Author: | Donnelley, M. Morgan, K. Siu, K. Farrow, N. Stahr, C. Boucher, R. Fouras, A. Parsons, D. |
Citation: | Scientific Reports, 2014; 4(3689):1-7 |
Publisher: | Nature Publishing Group |
Issue Date: | 2014 |
ISSN: | 2045-2322 2045-2322 |
Statement of Responsibility: | Martin Donnelley, Kaye S. Morgan, Karen K. W. Siu, Nigel R. Farrow, Charlene S. Stahr, Richard C. Boucher, Andreas Fouras & David W. Parsons |
Abstract: | To determine the efficacy of potential cystic fibrosis (CF) therapies we have developed a novel mucociliary transit (MCT) measurement that uses synchrotron phase contrast X-ray imaging (PCXI) to non-invasively measure the transit rate of individual micron-sized particles deposited into the airways of live mice. The aim of this study was to image changes in MCT produced by a rehydrating treatment based on hypertonic saline (HS), a current CF clinical treatment. Live mice received HS containing a long acting epithelial sodium channel blocker (P308); isotonic saline; or no treatment, using a nebuliser integrated within a small-animal ventilator circuit. Marker particle motion was tracked for 20 minutes using PCXI. There were statistically significant increases in MCT in the isotonic and HS-P308 groups. The ability to quantify in vivo changes in MCT may have utility in pre-clinical research studies designed to bring new genetic and pharmaceutical treatments for respiratory diseases into clinical trials. |
Keywords: | Biomedical engineering Pre-clinical studies Anatomy |
Rights: | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ © The Authors |
DOI: | 10.1038/srep03689 |
Published version: | http://dx.doi.org/10.1038/srep03689 |
Appears in Collections: | Aurora harvest 4 Paediatrics publications |
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hdl_81839.pdf | Published version | 630.37 kB | Adobe PDF | View/Open |
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