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https://hdl.handle.net/2440/81927
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dc.contributor.author | Richani, D. | - |
dc.contributor.author | Sutton-McDowall, M. | - |
dc.contributor.author | Frank, L. | - |
dc.contributor.author | Gilchrist, R. | - |
dc.contributor.author | Thompson, J. | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | Biology of Reproduction, 2014; 90(3):49-49 | - |
dc.identifier.issn | 0006-3363 | - |
dc.identifier.issn | 1529-7268 | - |
dc.identifier.uri | http://hdl.handle.net/2440/81927 | - |
dc.description.abstract | Oocyte in vitro maturation (IVM) is an assisted reproductive technology that involves the maturation of cumulus-oocyte-complexes (COCs) that are then capable of normal development. We have shown that epidermal growth factor (EGF)-like peptide signalling is perturbed in mouse COCs undergoing IVM when matured with FSH and/or EGF, but supplementation of IVM with EGF-like peptides amphiregulin or epiregulin improves oocyte developmental competence. Here we aimed to determine whether EGF-like peptides regulate COC metabolism. Immature 129/Sv mouse COCs underwent IVM with FSH, EGF, amphiregulin, epiregulin, betacellulin, or no treatment (control). Epiregulin significantly increased intra-oocyte FAD++ and REDOX ratio compared to FSH and control. Amphiregulin and epiregulin significantly increased the proportion of J-aggregates (from JC-1) in oocyte mitochondria compared to control, FSH or EGF, and this coupled with FAD++ and REDOX measures indicates greater mitochondrial activity. There were no differences in glucose consumption, lactate production, or glycolysis between COCs matured with FSH, EGF and EGF-like peptides. COCs matured with EGF or EGF-like peptides exhibited significantly higher mRNA expression of the hexosamine biosynthesis pathway (HBP) rate-limiting enzyme gene Gfpt2, Has2 expression, and global beta-O-linked glycosylation of proteins, compared to control or FSH, suggesting greater HBP activity. Our findings suggest that; (i) EGF-like peptides, particularly epiregulin, induce more oocyte mitochondrial activity than EGF or FSH, and (ii) EGF-like peptides and EGF induce greater HBP activity, enabling more hyaluronic acid synthesis and protein beta-O-linked glycosylation. These metabolic alterations may be a mechanism by which EGF-like peptides increase oocyte developmental competence. | - |
dc.description.statementofresponsibility | Dulama Richani, Melanie L. Sutton-McDowall, Laura A. Frank, Robert B. Gilchrist, and Jeremy G. Thompson | - |
dc.language.iso | en | - |
dc.publisher | Society for the Study of Reproduction | - |
dc.rights | Copyright 2014 by The Society for the Study of Reproduction. | - |
dc.source.uri | http://dx.doi.org/10.1095/biolreprod.113.115311 | - |
dc.subject | Cumulus cells | - |
dc.subject | Growth factors | - |
dc.subject | In vitro maturation (IVM) | - |
dc.subject | Metabolism | - |
dc.subject | Oocyte maturation | - |
dc.title | Effect of epidermal growth factor-like peptides on the metabolism of in vitro matured mouse oocytes and cumulus cells | - |
dc.type | Journal article | - |
dc.contributor.organisation | Robinson Institute | - |
dc.identifier.doi | 10.1095/biolreprod.113.115311 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Sutton-McDowall, M. [0000-0002-4121-0202] | - |
dc.identifier.orcid | Gilchrist, R. [0000-0003-1611-7142] | - |
dc.identifier.orcid | Thompson, J. [0000-0003-4941-7731] | - |
Appears in Collections: | Aurora harvest Obstetrics and Gynaecology publications |
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