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Type: Journal article
Title: Conservation and expression of piRNA pathway genes in male and female adult gonad of amniotes
Author: Lim, S.
Tsend-Ayush, E.
Kortschak, R.
Jacob, R.
Ricciardelli, C.
Oehler, M.
Grutzner, F.
Citation: Biology of Reproduction, 2013; 89(6):1-13
Publisher: Soc Study Reproduction
Issue Date: 2013
ISSN: 0006-3363
Organisation: Robinson Institute
Statement of
Shu Ly Lim, Enkhjargal Tsend-Ayush, R. Daniel Kortschak, Reuben Jacob, Carmela Ricciardelli, Martin K. Oehler, and Frank Grützner
Abstract: The PIWI-interacting RNA (piRNA) pathway is essential for germline development and transposable element repression. Key elements of this pathway are members of the piRNA-binding PIWI/Argonaute protein family and associated factors (e.g., VASA, MAELSTROM, and TUDOR domain proteins). PIWI-interacting RNAs have been identified in mouse testis and oocytes, but information about the expression of the different piRNA pathway genes, in particular in the mammalian ovary, remains incomplete. We investigated the evolution and expression of piRNA pathway genes in gonads of amniote species (chicken, platypus, and mouse). Database searches confirm a high level of conservation and revealed lineage-specific gain and loss of Piwi genes in vertebrates. Expression analysis in mammals shows that orthologs of Piwi-like (Piwil) genes, Mael (Maelstrom), Mvh (mouse vasa homolog), and Tdrd1 (Tudor domain-containing protein 1) are expressed in platypus adult testis. In contrast to mouse, Piwil4 is expressed in platypus and human adult testis. We found evidence for Mael and Piwil2 expression in mouse Sertoli cells. Importantly, we show mRNA expression of Piwil2, Piwil4, and Mael in oocytes and supporting cells of human, mouse, and platypus ovary. We found no Piwil1 expression in mouse and chicken ovary. The conservation of gene expression in somatic parts of the gonad and germ cells of species that diverged over 800 million yr ago indicates an important role in adult male and female gonad.
Keywords: amniotes; germ cells; gonads; Mael; Mvh; ovary; piRNA pathway genes; Piwi genes; PIWI-interacting genes; Tdrd1; testis
Rights: © 2013 by the Society for the Study of Reproduction, Inc.
RMID: 0020134318
DOI: 10.1095/biolreprod.113.111211
Grant ID:
Appears in Collections:Obstetrics and Gynaecology publications

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