Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/82048
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Type: Journal article
Title: Grain development in Brachypodium and other grasses: possible interactions between cell expansion, starch deposition, and cell-wall synthesis
Author: Trafford, K.
Haleux, P.
Henderson, M.
Parker, M.
Shirley, N.
Tucker, M.
Fincher, G.
Burton, R.
Citation: Journal of Experimental Botany, 2013; 64(16):5033-5047
Publisher: Oxford Univ Press
Issue Date: 2013
ISSN: 0022-0957
1460-2431
Statement of
Responsibility: 
Kay Trafford, Pauline Haleux, Marilyn Henderson, Mary Parker, Neil J. Shirley, Matthew R. Tucker, Geoffrey B. Fincher and Rachel A. Burton
Abstract: To explain the low levels of starch, high levels of (1,3;1,4)-β-glucan, and thick cell walls in grains of Brachypodium distachyon L. relative to those in other Pooideae, aspects of grain development were compared between B. distachyon and barley (Hordeum vulgare L.). Cell proliferation, cell expansion, and endoreduplication were reduced in B. distachyon relative to barley and, consistent with these changes, transcriptional downregulation of the cell-cycle genes CDKB1 and cyclin A3 was observed. Similarly, reduced transcription of starch synthase I and starch-branching enzyme I was observed as well as reduced activity of starch synthase and ADP-glucose pyrophosphorylase, which are consistent with the lowered starch content in B. distachyon grains. No change was detected in transcription of the major gene involved in (1,3;1,4)-β-glucan synthesis, cellulose synthase-like F6. These results suggest that, while low starch content results from a reduced capacity for starch synthesis, the unusually thick cell walls in B. distachyon endosperm probably result from continuing (1,3;1,4)-β-glucan deposition in endosperm cells that fail to expand. This raises the possibility that endosperm expansion is linked to starch deposition.
Keywords: Cell Wall; Poaceae; Seeds; Starch; Plant Proteins; Gene Expression Regulation, Plant; Brachypodium
Rights: © The Author 2013
RMID: 0020133601
DOI: 10.1093/jxb/ert292
Appears in Collections:Agriculture, Food and Wine publications

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