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Type: Journal article
Title: Development of an improved porcine embryo culture medium for cloning, transgenesis and embryonic stem cell isolation
Author: Beebe, L.
McIlfatrick, S.
Vassiliev, I.
Nottle, M.
Citation: Cloning and Transgenesis, 2013; 2(2):1-5
Publisher: OMICS Publishing Group
Issue Date: 2013
ISSN: 2168-9849
Statement of
Luke FS Beebe, Stephen M McIlfatrick, Ivan M Vassiliev and Mark B Nottle
Abstract: Work in our laboratory for more than two decades has focussed on the production of genetically modified pigs for xeno transplantion research. More recent work has focussed on the isolation of porcine embryonic stem cells to facilitate this as well as and other research applications. Central to this research has been the production of in vitro Produced (IVP) embryos. These embryos are produced using a twostep culture system based on NCSU23. This culture system which was developed by modifying energy substrate availability and concentrations and by adding non-essential and essential amino acids in a sequential manner. As a result of this work we have developed a culture system that better suits the changing metabolic needs of the pig embryo and produces embryos with relatively high developmental competence compared to the original formulation. These embryos can be used for a range of research applications including the isolation of embryonic stem cells.
Keywords: Cloning
Genetic Engineering
Rights: © 2013 Beebe LFS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI: 10.4172/2168-9849.1000107
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Appears in Collections:Aurora harvest
Obstetrics and Gynaecology publications

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