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Type: Journal article
Title: Characterisation of Walker 256 breast carinoma cells from two tumour cell banks as assessed using two models of secondary brain tumours
Author: Lewis, K.
Harford-Wright, E.
Vink, R.
Ghabriel, M.
Citation: Cancer Cell International, 2013; 13(5):1-12
Publisher: BioMed Central Ltd
Issue Date: 2013
ISSN: 1475-2867
Statement of
Kate M Lewis, Elizabeth Harford-Wright, Robert Vink and Mounir N Ghabriel
Abstract: Background: Metastatic brain tumours are a common end stage of breast cancer progression, with significant associated morbidity and high mortality. Walker 256 is a rat breast carcinoma cell line syngeneic to Wistar rats and commonly used to induce secondary brain tumours. Previously there has been the assumption that the same cancer cell line from different cell banks behave in a similar manner, although recent studies have suggested that cell lines may change their characteristics over time in vitro. Methods: In this study internal carotid artery injection and direct cerebral inoculation models of secondary brain tumours were used to determine the tumorigenicity of Walker 256 cells obtained from two cell banks, the American Type Culture Collection (ATCC), and the Cell Resource Centre for Medical Research at Tohoku University (CRCTU). Results: Tumour incidence and volume, plus immunoreactivity to albumin, IBA1 and GFAP, were used as indicators of tumorigenicity and tumour interaction with the host brain microenvironment. CRCTU Walker 256 cells showed greater incidence, larger tumour volume, pronounced blood–brain barrier disruption and prominent glial response when compared to ATCC cell line. Conclusions: These findings indicate that immortalised cancer cell lines obtained from different cell banks may have diverse characteristics and behaviour in vivo.
Keywords: Walker 256 cells
Brain metastases
Breast cancer
Animal models
Blood–brain barrier
Tumour banks
Glial reaction
Rights: © 2013 Lewis et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
DOI: 10.1186/1475-2867-13-5
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