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Type: Journal article
Title: Are sucrose transporter expression profiles linked with patterns of biomass partitioning in Sorghum phenotypes?
Author: Milne, R.
Byrt, C.
Patrick, J.
Grof, C.
Citation: Frontiers in Plant Science, 2013; 4(JUN):1-12
Publisher: Frontiers Research Foundation
Issue Date: 2013
ISSN: 1664-462X
Statement of
Ricky J. Milne, Caitlin S. Byrt, John W. Patrick and Christopher P. L. Grof
Abstract: Sorghum bicolor is a genetically diverse C4 monocotyledonous species, encompassing varieties capable of producing high grain yields as well as sweet types which accumulate soluble sugars (predominantly sucrose) within their stems to high concentrations. Sucrose produced in leaves (sources) enters the phloem and is transported to regions of growth and storage (sinks). It is likely that sucrose transporter (SUT) proteins play pivotal roles in phloem loading and the delivery of sucrose to growth and storage sinks in all Sorghum ecotypes. Six SUTs are present in the published Sorghum genome, based on the BTx623 grain cultivar. Homologues of these SUTs were cloned and sequenced from the sweet cultivar Rio, and compared with the publically available genome information. SbSUT5 possessed nine amino acid sequence differences between the two varieties. Two of the remaining five SUTs exhibited single variations in their amino acid sequences (SbSUT1 and SbSUT2) whilst the rest shared identical sequences. Complementation of a mutant Saccharomyces yeast strain (SEY6210), unable to grow up on sucrose as the sole carbon source, demonstrated that the Sorghum SUTs were capable of transporting sucrose. SbSUT1, SbSUT4, and SbSUT6 were highly expressed in mature leaf tissues and hence may contribute to phloem loading. In contrast, SbSUT2 and SbSUT5 were expressed most strongly in sinks consistent with a possible role of facilitating sucrose import into stem storage pools and developing inflorescences.
Keywords: Expression profiling
source–sink pathway
sucrose transporters
sucrose storage
Rights: Copyright: © 2013 Milne, Byrt, Patrick and Grof. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.
DOI: 10.3389/fpls.2013.00223
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Appears in Collections:Agriculture, Food and Wine publications
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