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Type: Journal article
Title: Accumulation of effector memory CD8⁺ T cells in nasal polyps
Other Titles: Accumulation of effector memory CD8(+) T cells in nasal polyps
Author: Pant, H.
Hughes, A.
Miljkovic, D.
Schembri, M.
Wormald, P.
Macardle, P.
Grose, R.
Zola, H.
Krumbiegel, D.
Citation: American Journal of Rhinology and Allergy, 2013; 27(5):117-126
Publisher: Ocean Side Publications Inc
Issue Date: 2013
ISSN: 1945-8924
Statement of
Harshita Pant, Amy Hughes, Dijana Miljkovic, Mark Schembri, Peter Wormald, Peta Macardle, Randall Grose, Heddy Zola,Doreen Krumbiegel
Abstract: BACKGROUND: T lymphocytes are prevalent in sinus mucosa and are implicated in chronic rhinosinusitis (CRS) pathogenesis. However, the major T-cell subpopulations, helper (CD4+) and cytotoxic (CD8+), have not been adequately examined in CRS. This study was designed to characterize human sinus mucosa and peripheral blood (PB) CD4+ and CD8+ T cells and their level of differentiation in CRS with nasal polyps (NPs), CRS without NPs, and control patients. METHODS: A prospective study was performed. Percentages of CD4+ and CD8+ T cells and their levels of differentiation were analyzed in sinus mucosa and PB by flow cytometry. Cell populations were defined as naive, central memory, effector memory, and effector T cells using cell surface markers CD45RA, CD62L, and CD27. The influence of coexisting allergy, sinus eosinophilic mucus (EM), and culture results were examined. RESULTS: In all patients, sinus mucosa had a lower percentage of CD4+ and a higher percentage of CD8+ T cells compared with PB. However, CRS with NPs (n = 86) had a significantly higher percentage of mucosal CD8+ T cells compared with CRS without NPs (n = 40) in control (n = 13) patients (p < 0.0001). Effector memory T cells were increased in sinuses compared with PB in all patients; however, the percentage of effector memory CD8+ T cells was greatest in CRS with NP mucosa (p = 0.002). Surprisingly coexisting allergy or culture results did not influence the mucosal T-cell phenotype. CRS with NP patients with sinus EM had a significantly higher percentage of mucosal CD8+ T cells. CONCLUSION: Sinus mucosa in CRS with NPs is characterized by a significant enrichment of CD8+ T cells and a relative deficiency of CD4+ T cells. The majority of NP CD8+ T cells had a terminally differentiated, mature, effector memory phenotype, which raises the question, whether these cells are pathogenic or appear as a consequence of inflammation, independent of the presence of allergy or positive microbial culture.
Keywords: Chronic rhinosinusitis
T cells
effector memory
nasal polyp
Rights: Copyright status unknown
DOI: 10.2500/ajra.2013.27.3958
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Appears in Collections:Aurora harvest
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