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Type: Journal article
Title: Expression of Caenorhabditis elegans RNA-directed RNA polymerase in transgenic Drosophila melanogaster does not affect morphological development
Author: Duan, G.
Saint, R.
Helliwell, C.
Behm, C.
Waterhouse, P.
Gordon, K.
Citation: Transgenic Research, 2010; 19(6):1121-1128
Publisher: Kluwer Academic Publ
Issue Date: 2010
ISSN: 0962-8819
Department: Division of the Deputy Vice-Chancellor and Vice-President (Research)
Statement of
Guowen Duan, Robert B. Saint, Chris A. Helliwell, Carolyn A. Behm, Peter M. Waterhouse, Karl H. J. Gordon
Abstract: Drosophila melanogaster, along with all insects and the vertebrates, lacks an RdRp gene. We created transgenic strains of Drosophila melanogaster in which the rrf-1 or ego-1 RdRp genes from C. elegans were placed under the control of the yeast GAL4 upstream activation sequence. Activation of the gene was performed by crossing these lines to flies carrying the GAL4 transgene under the control of various Drosophila enhancers. RT–PCR confirmed the successful expression of each RdRp gene. The resulting phenotypes indicated that introduction of the RdRp genes had no effect on D. melanogaster morphological development.
Keywords: RNA-directed RNA polymerase (RdRp)
RNA interference (RNAi)
MicroRNAs (miRNAs)
Rights: © Springer Science+Business Media B.V. 2010
DOI: 10.1007/s11248-010-9372-y
Appears in Collections:Aurora harvest 4
Molecular and Biomedical Science publications

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