Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/83014
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Type: Journal article
Title: Sex differences in mechanical allodynia: how can it be preclinically quantified and analyzed?
Author: Nicotra, L.
Tuke, S.
Grace, P.
Rolan, P.
Hutchinson, M.
Citation: Frontiers in Behavioral Neuroscience, 2014; 8(February):1-16
Publisher: Frontiers Research Foundation
Issue Date: 2014
ISSN: 1662-5153
1662-5153
Organisation: Institute for Photonics & Advanced Sensing (IPAS)
Statement of
Responsibility: 
Lauren Nicotra, Jonathan Tuke, Peter M. Grace, Paul E. Rolan and Mark R. Hutchinson
Abstract: Translating promising preclinical drug discoveries to successful clinical trials remains a significant hurdle in pain research. Although animal models have significantly contributed to understanding chronic pain pathophysiology, the majority of research has focused on male rodents using testing procedures that produce sex difference data that do not align well with comparable clinical experiences. Additionally, the use of animal pain models presents ongoing ethical challenges demanding continuing refinement of preclinical methods. To this end, this study sought to test a quantitative allodynia assessment technique and associated statistical analysis in a modified graded nerve injury pain model with the aim to further examine sex differences in allodynia. Graded allodynia was established in male and female Sprague Dawley rats by altering the number of sutures placed around the sciatic nerve and quantified by the von Frey test. Linear mixed effects modeling regressed response on each fixed effect (sex, oestrus cycle, pain treatment). On comparison with other common von Frey assessment techniques, utilizing lower threshold filaments than those ordinarily tested, at 1 s intervals, appropriately and successfully investigated female mechanical allodynia, revealing significant sex and oestrus cycle difference across the graded allodynia that other common behavioral methods were unable to detect. Utilizing this different von Frey approach and graded allodynia model, a single suture inflicting less allodynia was sufficient to demonstrate exaggerated female mechanical allodynia throughout the phases of dioestrus and pro-oestrus. Refining the von Frey testing method, statistical analysis technique and the use of a graded model of chronic pain, allowed for examination of the influences on female mechanical nociception that other von Frey methods cannot provide.
Keywords: Neuropathic pain; von Frey; sex; oestrus cycle
Description: Extent: 16 p.
Rights: © 2014 Nicotra, Tuke, Grace, Rolan and Hutchinson. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
RMID: 0030000050
DOI: 10.3389/fnbeh.2014.00040
Appears in Collections:IPAS publications

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