Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/83107
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Type: Journal article
Title: Fine mapping of the Rrs1 resistance locus against scald in two large populations derived from Spanish barley landraces
Author: Hofmann, K.
Silvar, C.
Casas, A.
Herz, M.
Buttner, B.
Gracia, M.
Contreras-Moreira, B.
Wallwork, H.
Igartua, E.
Schweizer, G.
Citation: Theoretical and Applied Genetics: international journal of plant breeding research, 2013; 126(12):3091-3102
Publisher: Springer
Issue Date: 2013
ISSN: 0040-5752
1432-2242
Statement of
Responsibility: 
Kerstin Hofmann, Cristina Silvar, Ana M. Casas, Markus Herz, Bianca Büttner, M. Pilar Gracia, Bruno Contreras‑Moreira, Hugh Wallwork, Ernesto Igartua, Günther Schweizer
Abstract: <h4>Key message</h4>In two Spanish barley landraces with outstanding resistance to scald, the Rrs1 Rh4 locus was fine mapped including all known markers used in previous studies and closely linked markers were developed. Scald, caused by Rhynchosporium commune, is one of the most prevalent barley diseases worldwide. A search for new resistance sources revealed that Spanish landrace-derived lines SBCC145 and SBCC154 showed outstanding resistance to scald. They were crossed to susceptible cultivar Beatrix to create large DH-mapping populations of 522 and 416 DH lines that were scored for disease resistance in the greenhouse using two R. commune isolates. To ascertain the pattern of resistance, parents and reference barley lines with known scald resistance were phenotyped with a panel of differential R. commune isolates. Subpopulations were genotyped with the Illumina GoldenGate 1,536 SNP Assay and a large QTL in the centromeric region of chromosome 3H, known to harbour several scald resistance genes and/or alleles, was found in both populations. Five SNP markers closest to the QTL were converted into CAPS markers. These CAPS markers, together with informative SSR markers used in other scald studies, confirmed the presence of the Rrs1 locus. The panel of differential scald isolates indicated that the allele carried by both donors was Rrs1 Rh4 . The genetic distance between Rrs1 and its flanking markers was 1.2 cM (11_0010) proximally and 0.9 cM (11_0823) distally, which corresponds to a distance of just below 9 Mbp. The number and nature of scald resistance genes on chromosome 3H are discussed. The effective Rrs1 allele found and the closely linked markers developed are already useful tools for molecular breeding programs and provide a good step towards the identification of candidate genes.
Keywords: Chromosomes, Plant
Ascomycota
Hordeum
DNA, Plant
Genetic Markers
Chromosome Mapping
Genetics, Population
Plant Diseases
Microsatellite Repeats
Genes, Plant
Immunity, Innate
Disease Resistance
Rights: © Springer-Verlag Berlin Heidelberg 2013
DOI: 10.1007/s00122-013-2196-4
Published version: http://dx.doi.org/10.1007/s00122-013-2196-4
Appears in Collections:Agriculture, Food and Wine publications
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