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Type: Journal article
Title: Genetic mapping and marker development for resistance of wheat against the root lesion nematode Pratylenchus neglectus
Author: Jayatilake, D.
Tucker, E.
Bariana, H.
Kuchel, H.
Edwards, J.
McKay, A.
Chalmers, K.
Mather, D.
Citation: BMC Plant Biology, 2013; 13(1):230-1-230-12
Publisher: BioMed Central Ltd.
Issue Date: 2013
ISSN: 1471-2229
Statement of
Dimanthi V Jayatilake, Elise J Tucker, Harbans Bariana, Haydn Kuchel, James Edwards, Alan C McKay, Ken Chalmers, and Diane E Mather
Abstract: BACKGROUND The Rlnn1 locus, which resides on chromosome 7A of bread wheat (Triticum aestivum L.) confers moderate resistance against the root lesion nematode Pratylenchus neglectus. Prior to this research, the exact linkage relationships of Rlnn1 with other loci on chromosome 7A were not clear and there were no simple codominant markers available for selection of Rlnn1 in wheat breeding. The objectives of the research reported here were to (1) develop an improved genetic map of the Rlnn1 region of chromosome 7A and (2) develop molecular markers that could be used in marker-assisted selection to improve resistance of wheat against P. neglectus. RESULTS A large-effect quantitative trait locus (QTL) for resistance against P. neglectus was genetically mapped using a population of Excalibur/Kukri doubled haploid lines. This QTL coincides in position with the rust resistance gene(s) Lr20/Sr15, the phytoene synthase gene Psy-A1 and 10 molecular markers, including five new markers designed using wheat-rice comparative genomics and wheat expressed sequence tags. Two of the new markers are suitable for use as molecular diagnostic tools to distinguish plants that carry Rlnn1 and Lr20/Sr15 from those that do not carry these resistance genes. CONCLUSIONS The genomic location of Rlnn1 was confirmed to be in the terminal region of the long arm of chromosome 7A. Molecular markers were developed that provide simple alternatives to costly phenotypic assessment of resistance against P. neglectus in wheat breeding. In Excalibur, genetic recombination seems to be completely suppressed in the Rlnn1 region.
Keywords: Root lesion nematode; Pratylenchus neglectus; Wheat; Molecular markers; Rlnn1; Lr20; Sr15; Psy-A1
Rights: © 2013 Jayatilake et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.
RMID: 0020136395
DOI: 10.1186/1471-2229-13-230
Appears in Collections:Agriculture, Food and Wine publications

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